Assessment of Anaerobic Bioremediation Potential in Hydrocarbon Contaminated Aquifers in Alberta, Canada

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dc.contributor.advisorGieg, Lisa Marie
dc.contributor.authorKharey, Gurpreet Singh
dc.contributor.committeememberHubert, Casey R. J.
dc.contributor.committeememberTurner, Raymond Joseph
dc.date2019-06
dc.date.accessioned2019-05-02T18:50:27Z
dc.date.available2019-05-02T18:50:27Z
dc.date.issued2019-04-30
dc.description.abstractAnaerobic biodegradation of hydrocarbon fuels (mono-aromatic and short chain alkane hydrocarbon) was demonstrated under both field and microcosm experiments, with detection of signature metabolites, fumarate addition genes, and microbial community composition to determine the potential of hydrocarbon-contaminated sites for bioremediation and assess the evidence of its past and/or present occurrence. It was determined here that the potential for bioremediation is unique to both the contaminated sites, but also to the hydrocarbon load present. Quantification of the fumarate addition genes using a newly-designed mixture of primers was done. These qPCR assays for assA and bssA abundances concluded that an upper limit to gene abundance is present according to hydrocarbon concentrations, of approximately 5 ppm hydrocarbon. Monitoring hydrocarbon loss from groundwater collected at these sites, paired with fumarate addition gene abundances concluded that the contaminated sites in question have the potential for bioremediation, but is limited with higher hydrocarbon concentrations.en_US
dc.identifier.citationKharey, G. S. (2019). Assessment of Anaerobic Bioremediation Potential in Hydrocarbon Contaminated Aquifers in Alberta, Canada (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/36433
dc.identifier.urihttp://hdl.handle.net/1880/110251
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjecthydrocarbonen_US
dc.subjectbioremediationen_US
dc.subjectqPCRen_US
dc.subjectenvironmental microbiologyen_US
dc.subjectfumarate additionen_US
dc.subjectanaerobic microbiologyen_US
dc.subject.classificationBiology--Cellen_US
dc.subject.classificationMicrobiologyen_US
dc.titleAssessment of Anaerobic Bioremediation Potential in Hydrocarbon Contaminated Aquifers in Alberta, Canadaen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
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