Involvement of pannexin-1 channels and the mitochondria permeability transition in ischemic neuronal death

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dc.contributor.advisorThompson, Roger John
dc.contributor.authorMa, Evelyn M.
dc.date.accessioned2017-12-18T22:19:54Z
dc.date.available2017-12-18T22:19:54Z
dc.date.issued2011
dc.descriptionBibliography: p. 66-74en
dc.descriptionA few pages are in colour.en
dc.descriptionIncludes copy of animal protocol approval. Original copy with original Partial Copyright Licence.en
dc.description.abstractStroke, the interruption in blood flow to the brain, initiates a neurotoxic cascade that leads to cell death. The aim of this thesis was to determine the role of Panx 1 channels in the neurotoxic cascade induced by ischemia in the rat hippocampus. Two approaches were taken: (1) the link between Panxl opening and MPT activation was investigated by monitoring dye efflux using live cell fluorescence microscopy, (2) the involvement of Panxl in cell death was determined by Western blot analysis of cleaved cytoskeletal protein, a-fodrin. Ischemia induced opening of the MPT was prevented by blocking calcium influx through Panxl channels. Panxl opening during ischemia also induced calpain proteolysis. This cleavage was prevented when either Panxl or the MPT were blocked. Therefore, opening of Panxl during ischemia activates the MPT and calpain proteases via calcium influx, indicating that Panxl channels induce neuronal death through mitochondria dysfunction and degradation of the cytoskeleton.
dc.format.extentxii, 75 leaves : ill. ; 30 cm.en
dc.identifier.citationMa, E. M. (2011). Involvement of pannexin-1 channels and the mitochondria permeability transition in ischemic neuronal death (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4167en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4167
dc.identifier.urihttp://hdl.handle.net/1880/105168
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleInvolvement of pannexin-1 channels and the mitochondria permeability transition in ischemic neuronal death
dc.typemaster thesis
thesis.degree.disciplineNeuroscience
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2025 627942875
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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