Fluid flow and Smad2 affects the response of vascular endothelial cells in vitro and in vivo

Simple item page
atmire.migration.oldid4166
dc.contributor.advisorRinker, Kristina D.
dc.contributor.authorTamez-Vielma, Linda Selene
dc.contributor.committeememberMoore, Randy
dc.contributor.committeememberDi Martino, Elena
dc.contributor.committeememberProud, David
dc.contributor.committeememberUngrin, Mark
dc.contributor.committeememberMahinpey, Nader
dc.date.accessioned2016-02-05T18:26:14Z
dc.date.available2016-02-05T18:26:14Z
dc.date.issued2016-02-05
dc.date.submitted2016en
dc.description.abstractHuman aortic endothelial cells (HAECs) have been observed to respond to fluid flow and shear stress by activating different signalling molecules both in vitro and in vivo. An important example of these flow-activated molecules is Smad2. Smad2 is a signalling molecule and transcription factor that has shown to be indispensable for the maintenance of vascular integrity. The aim of this study was to understand the effect of shear stress and Smad2 knockdown on endothelial gene expression. HAEC were transfected with Smad2 siRNA, and exposed to steady laminar shear stress (10 dyne/cm2). Our results showed that Smad2 siRNA and shear stress significantly up-regulated genes involved in atherosclerosis, heart dysfunction, and angiogenesis. Furthermore, Smad2 siRNA had a negative impact on athero-protective genes under static conditions. This is the first reported Smad2 siRNA gene expression profile of endothelial cells. Our findings suggest that Smad2 may a have a protective role against cardiovascular diseases.en_US
dc.identifier.citationTamez-Vielma, L. S. (2016). Fluid flow and Smad2 affects the response of vascular endothelial cells in vitro and in vivo (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26194en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26194
dc.identifier.urihttp://hdl.handle.net/11023/2847
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBioinformatics
dc.subjectBiology--Cell
dc.subjectGenetics
dc.subjectBiology--Molecular
dc.subjectMedicine and Surgery
dc.subject.classificationSignalling pathwaysen_US
dc.subject.classificationGene expression profileen_US
dc.subject.classificationWestern Bloten_US
dc.subject.classificationsiRNAen_US
dc.titleFluid flow and Smad2 affects the response of vascular endothelial cells in vitro and in vivo
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Collections
Open Theses and Dissertations