The role of extracellular DNA in neutrophil extracellular traps

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atmire.migration.oldid1421
dc.contributor.advisorLewenza, (William) Shawn
dc.contributor.authorHalverson, Tyler William Ralph
dc.date.accessioned2013-09-25T18:47:11Z
dc.date.available2014-09-26T07:00:20Z
dc.date.issued2013-09-25
dc.date.submitted2013en
dc.description.abstractPseudomonas aeruginosa is an opportunistic pathogen and the primary pathogen in lung infections in patients with Cystic Fibrosis (CF). Neutrophils are recruited to the site of infection to clear the invading bacteria by phagocytosis and neutrophil extracellular trap (NET) formation. NETs are composed of an extracellular lattice of genomic DNA enmeshed with various antimicrobial components normally found within the neutrophil. NETs trap and kill bacteria; however, the killing attributes of NETs are poorly understood. Here we show that the DNA is an important antimicrobial component of NETs. P. aeruginosa senses and responds to DNA in NETs by inducing genes required to survive NET killing. P. aeruginosa uses various surface modifications for NET resistance and secreted enzymes to degrade and neutralize NETs. These defensive strategies promote survival during neutrophil encounters and illustrate a dynamic example of innate immune evasion by P. aeruginosa.en_US
dc.description.embargoterms12 monthsen_US
dc.identifier.citationHalverson, T. W. (2013). The role of extracellular DNA in neutrophil extracellular traps (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28324en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28324
dc.identifier.urihttp://hdl.handle.net/11023/1040
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectSciences
dc.subjectMicrobiology
dc.subject.classificationPseudomonas aeurginosaen_US
dc.subject.classificationNeutrophil extracellular trapsen_US
dc.subject.classificationextracellular DNAen_US
dc.subject.classificationNeutrophilsen_US
dc.subject.classificationCystic Fibrosisen_US
dc.subject.classificationantimicrobial resistanceen_US
dc.subject.classificationsurface modificationsen_US
dc.subject.classificationdefense mechanismsen_US
dc.subject.classificationhost-pathogen interactionsen_US
dc.titleThe role of extracellular DNA in neutrophil extracellular traps
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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