Utilization of plant Puf RNA-binding proteins to manipulate endogenous mRNA physiology

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dc.contributor.advisorMuench, Douglas G.
dc.contributor.authorWen, Xin
dc.contributor.committeememberHansen, David V.
dc.contributor.committeememberBarrette-Ng, Isabelle H.
dc.date2019-06
dc.date.accessioned2019-02-20T16:14:46Z
dc.date.available2019-02-20T16:14:46Z
dc.date.issued2019-02-01
dc.description.abstractRNA-binding proteins have an important role in the regulation of post-transcriptional gene expression. The Puf (Pumilio) family of RNA-binding proteins bind to RNAs in a sequence specific manner. The RNA-binding domain of canonical Puf proteins (the Pumilio homology domain, PUM-HD) consists of eight Puf repeats. Each Puf repeat binds to RNA in a modular fashion, so that one Puf repeat binds to a single nucleotide. The nucleotide binding recognition code of Puf repeats has been well characterized. This one repeat:one nucleotide recognition code has allowed for the alteration of Puf repeats so that the PUM-HD can bind specific RNA targets. These altered Puf repeats have been fused to effector domains so that fusion proteins can affect RNA physiology in the cell. The aim of this thesis was to fuse an Arabidopsis Puf RNA-binding protein with unique binding characteristics to two types of effector domains. These fusion proteins were expressed in onion epidermal cells to determine if they could alter the stability or translation of a reporter mRNA. This research provides foundational evidence that highlights the potential for the utility of plant Puf proteins to alter gene expression for applied purposesen_US
dc.identifier.citationWen, X. (2019). Utilization of plant Puf RNA-binding proteins to manipulate endogenous mRNA physiology (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/36146
dc.identifier.urihttp://hdl.handle.net/1880/109909
dc.language.isoenen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.subjectAPUM23en_US
dc.subjectPuf proteinsen_US
dc.subjectRNA-binding proteinsen_US
dc.subjectProtein engineeringen_US
dc.subject.classificationEducation--Sciencesen_US
dc.titleUtilization of plant Puf RNA-binding proteins to manipulate endogenous mRNA physiologyen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
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