Comparisons of EmrE Purification Methods
| atmire.migration.oldid | 900 | |
| dc.contributor.advisor | Turner, Raymond | |
| dc.contributor.author | Chew, Raymond | |
| dc.date.accessioned | 2013-04-30T22:36:34Z | |
| dc.date.available | 2013-06-10T07:00:46Z | |
| dc.date.issued | 2013-04-30 | |
| dc.date.submitted | 2013 | en |
| dc.description.abstract | Due to the difficulties of membrane protein expression, isolation, and solubilisation, purification yields are typically lower than most soluble proteins. A common tactic to increase yield is the use of affinity tags for co-purification of the attached protein. EmrE, a small bacterial multidrug efflux protein was examined to characterize changes in structure and function due to a His6 affinity tag. Fluorescence spectroscopy revealed the presence of a relatively hydrophobic fold in tagged EmrE as well as slower solvent dynamics within the folded protein. In regards to functionality, the untagged EmrE displayed higher ligand binding affinities to ethidium and methyl viologen in vitro and also higher transport activity in vivo. | en_US |
| dc.identifier.citation | Chew, R. (2013). Comparisons of EmrE Purification Methods (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25377 | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/25377 | |
| dc.identifier.uri | http://hdl.handle.net/11023/661 | |
| dc.language.iso | eng | |
| dc.publisher.faculty | Graduate Studies | |
| dc.publisher.institution | University of Calgary | en |
| dc.publisher.place | Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | |
| dc.subject | Biochemistry | |
| dc.subject.classification | Protein purification, fluorescence, EmrE, SMR, quaternary cationic compounds, ligand binding | en_US |
| dc.title | Comparisons of EmrE Purification Methods | |
| dc.type | master thesis | |
| thesis.degree.discipline | Biological Sciences | |
| thesis.degree.grantor | University of Calgary | |
| thesis.degree.name | Master of Science (MSc) | |
| ucalgary.item.requestcopy | true |