Development of the liver in the chicken embryo.  I. Hepatic cords and sinusoids

Cavey, Michael J.; Wong, Gene K.

Development of the liver in the chicken embryo. I. Hepatic cords and sinusoids

dc.contributor.author	Cavey, Michael J.	en
dc.contributor.author	Wong, Gene K.	en
dc.contributor.department	Biological Sciences	en
dc.contributor.faculty	Faculty of Science	en
dc.contributor.institution	University of Calgary	en
dc.date.accessioned	2006-08-14T20:02:06Z
dc.date.available	2006-08-14T20:02:06Z
dc.date.issued	1992
dc.description	Copyright held by publisher	en
dc.description.abstract	Hemopoiesis in the liver of the chicken embryo begins on day 7 of incubation (Hamburger and Hamilton Stage 30) and peaks on day 14 (Stage 40). During this time frame, the differentiation of hepatic cells was examined by light microscopy, transmission and scanning electron microscopy, and morphometry. The avian liver is a closely packed mass of dendriform cords and discontinuous sinusoids. Hepatocytes are pyramidal in shape, and they ring the bile canaliculi which run through the centers of the cords. Semithin sections, made possible by infiltration and embedding in glycol methacrylate, were stained with hematoxylin and eosin to assess the general architecture of the organ and the lipid content of the hepatocytes and by the periodic acid-Schiff reaction and hematoxylin to visualize the cytoplasmic stores of glycogen. The number of hepatocytes with demonstrable glycogen fluctuates erratically in early hemopoiesis, and the proportion of glycogen-containing cells progressively increases as hemopoiesis climbs to a peak. Most differentiating hepatocytes are devoid of lipid droplets until Stages 39 and 40. From Stage 30 to 35, hepatocyte volume falls to its lowest value. Subsequently (Stages 36 to 40), cell volume increases and hepatocytes achieve a relatively uniform size. Ultrastructural changes in the differentiating hepatocytes, including alterations to the mitochondria, endoplasmic reticulum, and Golgi apparatus, are documented. These morphological and morphometric findings on the prehepatocyte population and hepatic vasculature cover 2 of the 3 elements deemed critical to hepatic hemopoiesis in many vertebrates. o 1992 Wiley-Liss, Inc.	en
dc.description.refereed	yes	en
dc.format.extent	2079727 bytes
dc.format.mimetype	application/pdf
dc.identifier.citation	GENE K. WONG AND MICHAEL J. CAVEY "Development of the Liver in the Chicken Embryo. I. HEPATIC CORDS AND SlNUSOlDS" THE ANATOMICAL RECORD 234555-567 (1992)	en
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/35099
dc.identifier.issn	1552-4884
dc.identifier.uri	http://hdl.handle.net/1880/43417
dc.language.iso	en	en
dc.publisher	John Wiley & Sons, Inc.	en
dc.publisher.url	http://www3.interscience.wiley.com/cgi-bin/jhome/28243
dc.subject	Biology	en
dc.subject.other	Capillaries	en
dc.subject.other	Chick embryo	en
dc.subject.other	Vascular endothelium	en
dc.subject.other	Hematopoiesis	en
dc.subject.other	Kupffer cells	en
dc.subject.other	Liver	en
dc.subject.other	Microscopy	en
dc.subject.other	Morphogenesis	en
dc.title	Development of the liver in the chicken embryo. I. Hepatic cords and sinusoids	en
dc.type	journal article