Response to Diet-Induced Obesity Produces Time-dependent Induction and Progression of Metabolic Osteoarthritis in Rat Knees

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dc.contributor.authorHerzog, Walter
dc.contributor.authorCollins, Kelsey
dc.contributor.authorHart, David A,
dc.contributor.authorReimer, Raylene A.
dc.contributor.authorSeerattan, Ruth A.
dc.date.accessioned2016-06-21T21:41:48Z
dc.date.available2016-06-21T21:41:48Z
dc.date.issued2016
dc.description.abstractObesity, and corresponding chronic-low grade inflammation, is associated with the onset and progression of knee OA. The origin of this inflammation is poorly understood. Here, the effect of high fat, high sucrose (HFS) diet induced obesity (DIO) on local (synovial fluid) and systemic (serum) inflammation is evaluated after a 12-week obesity induction and a further 16-week adaptation period. For 12-weeks of obesity induction, n=40 DIO male Sprague-Dawley rats consumed a HFS diet while the control group (n=14) remained on chow. DIO rats were allocated to prone (DIO-P, top 33% based on weight change) or resistant (DIO-R, bottom 33%) groups at 12-weeks. Animals were euthanized at 12- and after an additional 16-weeks on diet (28-weeks). At sacrifice, body composition and knee joints were collected and assessed. Synovial fluid and sera were profiled using cytokine array analysis. At 12-weeks, DIO-P animals demonstrated increased Modified Mankin Scores compared to DIO-R and Chow (p=0.026), and DIO-R had higher Mankin scores compared to Chow (p=0.049). While numerous systemic and limited synovial fluid inflammatory markers were increased at 12-weeks in DIO animals compared to Chow, by 28-weeks there were limited systemic differences but marked increases in local synovial fluid inflammatory marker concentrations. Metabolic OA may manifest from an initial systemic inflammatory disturbance. 12-weeks of obesity induction leads to a unique inflammatory profile and induction of metabolic OA which is altered after a further 16-weeks of obesity and HFS diet intake, suggesting that obesity is a dynamic, progressive process.en_US
dc.description.refereedYesen_US
dc.identifier.citationCollins, K. H., Hart, D. A., Reimer, R. A., Seerattan, R. A., & Herzog, W. (2015). Response to diet‐induced obesity produces time‐dependent induction and progression of metabolic osteoarthritis in rat knees. Journal of Orthopaedic Research.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/29062
dc.identifier.urihttp://hdl.handle.net/1880/51438
dc.language.isoenen_US
dc.publisherJournal of Orthopaedic Researchen_US
dc.publisher.departmentHuman Performance Laboratoryen_US
dc.publisher.facultyKinesiologyen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.subjectmetabolic osteoarthritisen_US
dc.subjectsynovial fluiden_US
dc.subjectraten_US
dc.subjectsystemic inflammationen_US
dc.subjectknee jointen_US
dc.titleResponse to Diet-Induced Obesity Produces Time-dependent Induction and Progression of Metabolic Osteoarthritis in Rat Kneesen_US
dc.typejournal article
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