Gene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease”

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atmire.migration.oldid1934
dc.contributor.advisorDe Buck, Jeroen
dc.contributor.advisorBarkema, Herman
dc.contributor.advisorGhosh, Subrata
dc.contributor.advisorGuan, LeLuo
dc.contributor.authorDavid, Joel
dc.date.accessioned2014-02-05T18:44:55Z
dc.date.available2014-03-15T07:00:21Z
dc.date.issued2014-02-05
dc.date.submitted2013en
dc.description.abstractCurrent diagnostic tools lack sensitivity to detect JD early after infection. Hence, this study aimed to find biomarkers for early diagnosis of JD. An infection trial was set up with a high dose (n=5) and low dose (n=5) oral challenge with Mycobacterium avium subsp. paratuberculosis at 2 weeks of age and 5 non-infected control animals. Whole blood was collected at a 3-month interval (3, 6, 9, 12 and 15 months) for gene expression analysis using Affymetrix® GeneChip® Bovine Genome Array on samples obtained at 3, 6 and 9 months after infection. Microarray findings were confirmed using qPCR and longitudinal assays profiling transcripts over 15-month period was carried out. This study found differential gene expression at each sampled time point and found transcripts for potential biomarker use. CD46 was upregulated and BOLA and BNBD9-like genes were downregulated over the entire 15-months. Overall immune response was inhibited in MAP-challenged animals.en_US
dc.identifier.citationDavid, J. (2014). Gene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease” (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26265en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26265
dc.identifier.urihttp://hdl.handle.net/11023/1368
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subject.classificationJohne’s diseaseen_US
dc.subject.classificationMycobacterium avium subspecies paratuberculosisen_US
dc.subject.classificationTranscriptomicen_US
dc.subject.classificationBiomarkersen_US
dc.titleGene Expression Study Of Mycobacterium avium subspecies paratuberculosis Infected Cows – “A road to identify transcripts that could serve as biomarkers for early diagnosis of Johne’s disease”
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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List of differentially expressed genes at 3 months after infection
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Canonical pathways associated with ELISA dataset at 3 months
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