The thermosensory diguanylate cyclase (tdcA)

Simple item page
atmire.migration.oldid4398
dc.contributor.advisorHarrison, Joe
dc.contributor.authorRandall, Trevor Ethan
dc.contributor.committeememberStorey, Douglas
dc.contributor.committeememberFraser, Marie
dc.date.accessioned2016-05-06T18:32:02Z
dc.date.available2016-05-06T18:32:02Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractBiofilm production in Pseudomonas aeruginosa is regulated by the intracellular secondary messenger cyclic diguanylate (c-di-GMP). C-di-GMP is produced by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). C-di-GMP controls motility, biofilm formation and virulence factor production in many bacteria. While much is known about how DGCs and PDEs synthesize and degrade c-di-GMP, respectively, little is understood about how environmental stimuli modulate enzyme activity. We identified the thermosensing diguanylate cyclase (tdcA) which allows for the thermoregulation of biofilm formation in P. aeruginosa. Activity assays revealed that TdcA activity increased ~35 fold as the temperature of the system increased from environmental to body temperature. Further, analysis revealed that TdcA acts as a thermostat, allowing for TdcA activity to be modulated by temperature. Our results demonstrate that TdcA is a DGC that acts as a molecular thermostat in P. aeruginosa.en_US
dc.identifier.citationRandall, T. E. (2016). The thermosensory diguanylate cyclase (tdcA) (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28399en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28399
dc.identifier.urihttp://hdl.handle.net/11023/2991
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subject.classificationBiochemistryen_US
dc.subject.classificationSignal Transductionen_US
dc.subject.classificationc-di-GMPen_US
dc.subject.classificationPseudomonas aeruginosaen_US
dc.subject.classificationthermosensationen_US
dc.titleThe thermosensory diguanylate cyclase (tdcA)
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Collections
Open Theses and Dissertations