A heat-activated protein expression system for biotechnology

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dc.contributor.advisorHarrison, Joe J.
dc.contributor.authorFournier, Nicolas
dc.contributor.committeememberChua, Gordon
dc.contributor.committeememberFacchini, Peter J.
dc.date2020-02
dc.date.accessioned2019-12-03T23:52:08Z
dc.date.available2019-12-03T23:52:08Z
dc.date.issued2019-12
dc.description.abstractThe thermosensory diguanylate cyclase (TdcA) enzyme found in the Pseudomonas aeruginosa strain CF39S produces cyclic diguanylate (c-di-GMP) in response to increases in temperature. The application of TdcA in a heat-activated protein expression system was explored as an alternative to conventional induction systems. C-di-GMP-dependent gene expression measurements were conducted to investigate gene expression levels in Pseudomonas species in the presence of TdcA. An E. coli K12 mutant library was also used to investigate temperature-dependent gene expression levels for the development of a heat-activated protein expression system. Results demonstrated the thermostatted activity of TdcA in Pseudomonas species, while multiple molecular determinants were found to contribute to temperature-dependent biofilm formation and c-di-GMP-dependent gene expression levels in E. coli K12 species. Of these determinants, the c-di-GMP specific phosphodiesterase PdeO was identified as a significant contributor. Further investigation into the applicability of heat-activated expression systems would be of great benefit to the biotechnology sector.en_US
dc.identifier.citationFournier, N. (2019). A heat-activated protein expression system for biotechnology (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37319
dc.identifier.urihttp://hdl.handle.net/1880/111308
dc.language.isoengen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectc-di-GMPen_US
dc.subjectmicrobiologyen_US
dc.subject.classificationMicrobiologyen_US
dc.titleA heat-activated protein expression system for biotechnologyen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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