Detection of Antibodies Against the Open Reading Frame Three Protein of Porcine Circovirus 2 in Pigs

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atmire.migration.oldid2232
dc.contributor.advisorCzub, Markus
dc.contributor.authorHawkes, Aaron
dc.date.accessioned2014-07-10T22:45:12Z
dc.date.available2014-11-17T08:00:37Z
dc.date.issued2014-07-10
dc.date.submitted2014en
dc.description.abstractThe swine pathogen porcine circovirus 2 (PCV2) is the primary etiological agent of a syndrome that is damaging to the swine industry. The PCV2 genome contains three major open reading frames (ORFs) and ORF3 appears to play a role in PCV2-related pathology and dissemination. No data have been published confirming that PCV2ORF3 protein is expressed in PCV2-infected swine. Here it is hypothesized that αPCV2ORF3 antibodies are present in the sera of most adult swine. Swine sera were screened for αPCV2ORF3 antibodies, the presence of which would imply PCV2ORF3 expression. About 90% of the tested sera from weaned swine appear to have at least some reactivity to rPCV2ORF3, implying that PCV2ORF3 is expressed in vivo. Serum reactivity to PCV2ORF3 did not correlate with PCV2 viral load or vaccination status. These results do not indicate or rule out any particular biological role for PCV2ORF3.en_US
dc.identifier.citationHawkes, A. (2014). Detection of Antibodies Against the Open Reading Frame Three Protein of Porcine Circovirus 2 in Pigs (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25599en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25599
dc.identifier.urihttp://hdl.handle.net/11023/1620
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectVeterinary Science
dc.subjectVirology
dc.subjectBiochemistry
dc.subject.classificationporcine circovirusen_US
dc.subject.classificationrecombinant expressionen_US
dc.subject.classificationSerologyen_US
dc.titleDetection of Antibodies Against the Open Reading Frame Three Protein of Porcine Circovirus 2 in Pigs
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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