Differential sorting of ephrin ligands in a polarized cell line

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dc.contributor.advisorRobbins, Stephen
dc.contributor.authorBox, Alan Kirby
dc.date.accessioned2005-08-16T17:37:10Z
dc.date.available2005-08-16T17:37:10Z
dc.date.issued2004
dc.descriptionBibliography: p. 137-169en
dc.descriptionSome pages are in colour.en
dc.description.abstractThe Eph family of receptor is the largest family of receptor tyrosine kinases known. The ephrins are the membrane tethered ligands for this system. The ephrins are divided into two classes: the glycosylphosphatidylinositol anchored A-class ephrins, and the transmembrane B-class ephrins. Both classes of ephrins are associated with lipid rafts. The ephrins were expressed in MDCK cells, a polarized epithelial cell line that uses lipid rafts in sorting. Ephrin-A5 is apically sorted while ephrin-Bl is basolaterally sorted in this system as judged by immunofluorescence and Western blotting. The C-terminus of ephrin-Bl is not involved in its sorting in MDCK cells. The two classes of ephrins are differentially sensitive to Triton X-IOO and Brij 58, suggesting that they may be localized to different lipid rafts. The presence of ephrins in MDCK cells alters the actin cytoskeleton, which may explain the enhanced response to HGF/SF in this system.en
dc.format.extentxiv, 169 leaves : ill. ; 30 cm.en
dc.identifier.citationBox, A. K. (2004). Differential sorting of ephrin ligands in a polarized cell line (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/22245en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/22245
dc.identifier.isbn0612975266en
dc.identifier.lccAC1 .T484 2004 B69en
dc.identifier.urihttp://hdl.handle.net/1880/42188
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleDifferential sorting of ephrin ligands in a polarized cell line
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 1491 520492008
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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