In Situ Chondrocyte Mechanics and Mechanobiology

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atmire.migration.oldid1280
dc.contributor.advisorHerzog, Walter
dc.contributor.authorMadden, Ryan Matthew Jerome
dc.date.accessioned2013-09-04T16:46:01Z
dc.date.available2013-11-12T08:00:12Z
dc.date.issued2013-09-04
dc.date.submitted2013en
dc.description.abstractChondrocyte metabolism is stimulated by mechanical loading and is associated with structuralchanges in the cartilage extracellular matrix (ECM). Calcium signaling is an initial step in the biological response of cells to mechanical loading. The purpose of this work was to measure local ECM and chondrocyte deformations for a range of tissue strains and to relate the measured deformations to chondrocyte calcium signaling in intact cartilage attached to its native bone. We observed that: 1. Chondrocytes are protected from excessive deformation when cartilage is subjected to extreme compressive strains, likely due to the local extra- and pericellular matrices; 2. Chondrocyte calcium signaling is strongly correlated to compressive loading magnitude and the local ECM strains within the tissue; and, 3. Chondrocyte mechanobiology varies topographically within a joint. These results provide new insights into the relationship between compressive mechanical loading, the resulting tissue and cell deformations, and the calcium signaling response of the chondrocytes.en_US
dc.identifier.citationMadden, R. M. (2013). In Situ Chondrocyte Mechanics and Mechanobiology (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26553en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26553
dc.identifier.urihttp://hdl.handle.net/11023/907
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectCell
dc.subjectBiomedical
dc.subject.classificationArticular cartilageen_US
dc.subject.classificationChondrocyteen_US
dc.subject.classificationMechanobiologyen_US
dc.subject.classificationMechanotransductionen_US
dc.subject.classificationCalcium signalingen_US
dc.subject.classificationLarge deformationen_US
dc.titleIn Situ Chondrocyte Mechanics and Mechanobiology
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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