Quantification of HIV-1 Proviral DNA Forms in Gut Tissues Using Real-Time PCR

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atmire.migration.oldid3637
dc.contributor.advisorvan Marle, Guido
dc.contributor.authorBahafzallah, Laila
dc.date.accessioned2015-09-29T17:14:39Z
dc.date.available2015-11-20T08:00:43Z
dc.date.issued2015-09-29
dc.date.submitted2015en
dc.description.abstractPrevious studies have shown that infection of gut tissues (esophagus-stomach-duodenum-colon) by HIV is compartmentalized with increased HIV-1 diversity and selection for drug resistance in the colon. We looked at the kinetics of HIV-1 in the gut tissues obtained from infected patients receiving ART (i.e AZT or ddI). We established real- time PCR protocols to detect HIV-1 proviral DNA forms. The total HIV-1 proviral DNA was the most frequent form found in the gut tissues. 2-LTR proviral DNA was also detected but in low levels which might indicate low viral replication. HIV-1 latent infection was also detected in the gut tissues as represented by the integrated proviral DNA levels. The levels detected of the proviral DNA forms varied between gut tissues, visits and patients, and no difference or correlation were found for patients receiving AZT or ddI.en_US
dc.identifier.citationBahafzallah, L. (2015). Quantification of HIV-1 Proviral DNA Forms in Gut Tissues Using Real-Time PCR (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27495en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27495
dc.identifier.urihttp://hdl.handle.net/11023/2542
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subject.classificationHIV-1 proviral DNA formsen_US
dc.subject.classification2-LTR proviral DNAen_US
dc.subject.classificationIntegrated proviral DNAen_US
dc.subject.classificationGut Tissuesen_US
dc.titleQuantification of HIV-1 Proviral DNA Forms in Gut Tissues Using Real-Time PCR
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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