Functional Genomics and Metabolite Profiling as Tools for Alkaloid Biosynthetic Gene Discovery

Simple item page
atmire.migration.oldid3907
dc.contributor.advisorFacchini, Peter James
dc.contributor.authorDinsmore, Donald Reed
dc.contributor.committeememberMoorhead, Gregory
dc.contributor.committeememberSchriemer, David
dc.contributor.committeememberDerksen, Darren
dc.date.accessioned2015-12-16T17:55:03Z
dc.date.available2015-12-16T17:55:03Z
dc.date.issued2015-12-16
dc.date.submitted2015en
dc.description.abstractThe benzylisoquinoline alkaloids (BIAs) are diverse group of plant specialized metabolites found in the families Papaveracea, Ranunculaceae, Berberidaceae and Menispermaceae. Plants remain the only commercial source for BIAs and their biosynthesis is poorly understood. O-methyltransferases (OMTs) are wide spread in BIA biosynthesis. Putative OMTs were found in stem and root Next-Generation Sequencing transcriptomic databases. Putative OMT cDNAs were isolated from Papaver somniferum and commercially synthesized. Recombinant protoberberine 2-O-methyltransferase (2OMT) was heterologously expressed in Escherichia coli and assayed. 2OMT demonstrated the 2-O-methylation of protoberberine alkaloids and the 7-O-methylation of simple BIAs. The substrate range and tissue specific expression of 2OMT suggest its in vivo role is converting (S)-cheilanthifoline to (S)-sinactine. A LC-MS based targeted alkaloid profiling of twenty BIA producing species from the families Papaveracea, Ranunculaceae, Berberidaceae and Menispermaceae was conducted.en_US
dc.identifier.citationDinsmore, D. R. (2015). Functional Genomics and Metabolite Profiling as Tools for Alkaloid Biosynthetic Gene Discovery (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26246en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26246
dc.identifier.urihttp://hdl.handle.net/11023/2686
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBioinformatics
dc.subjectBotany
dc.subjectGenetics
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subjectPlant Physiology
dc.subjectChemistry--Analytical
dc.subjectBiochemistry
dc.subject.classificationPlantsen_US
dc.subject.classificationMetabolismen_US
dc.subject.classificationMass spectrometryen_US
dc.subject.classificationEnzymeen_US
dc.subject.classificationOpium poppyen_US
dc.titleFunctional Genomics and Metabolite Profiling as Tools for Alkaloid Biosynthetic Gene Discovery
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Collections
Open Theses and Dissertations