Origin and diversification of tissue-resident fibroblasts

Ma, Roger

Origin and diversification of tissue-resident fibroblasts

dc.contributor.advisor	Huang, Peng
dc.contributor.author	Ma, Roger
dc.contributor.committeemember	Grewal, Savraj
dc.contributor.committeemember	Mains, Paul
dc.date	2021-06
dc.date.accessioned	2021-03-18T18:54:57Z
dc.date.available	2021-03-18T18:54:57Z
dc.date.issued	2021-03-12
dc.description.abstract	Every cell within a multicellular organism contains the same genome and ultimately originates from a single cell. Despite this, a vast array of cell types is created during development. Understanding how a single progenitor can differentiate into distinct cell types is one of the fundamental questions in developmental biology. For example, while it is known that the somites generate much of the axial body, the specific mechanisms of how this occurs remains poorly understood. In this thesis, I use the zebrafish sclerotome as a model to study how different cell types can be generated from a common pool of progenitor cells. During development, the sclerotome is subdivided from the somite and gives rise to the axial skeleton, cartilage and tendons. In Chapter 2, I characterize sclerotome development in zebrafish and identify a novel dorsal sclerotome domain unique in zebrafish. Active hedgehog signaling is required for the migration of and maintenance of sclerotome-derived cells. Lineage analysis reveals that the sclerotome progenitors give rise to tenocytes (tendon fibroblasts) in a stereotypical manner. In Chapter 3, I ask how sclerotome progenitors diversify into multiple fibroblast subtypes. Using single cell lineage analysis, reveals that the sclerotome is multipotent and generates a variety of fibroblasts in the zebrafish trunk. BMP signaling is required for the generation and maintenance of a subset of sclerotome-derived fibroblasts, the fin mesenchymal cells. Together, my work shows that the zebrafish sclerotome is the embryonic origin of a diverse population of tissue-resident fibroblasts and can be a good model to study cell type diversification.	en_US
dc.identifier.citation	Ma, R. (2021). Origin and diversification of tissue-resident fibroblasts (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.	en_US
dc.identifier.doi	http://dx.doi.org/10.11575/PRISM/38676
dc.identifier.uri	http://hdl.handle.net/1880/113153
dc.language.iso	eng	en_US
dc.publisher.faculty	Cumming School of Medicine	en_US
dc.publisher.institution	University of Calgary	en
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.	en_US
dc.subject.classification	Biology	en_US
dc.subject.classification	Biology--Cell	en_US
dc.subject.classification	Genetics	en_US
dc.subject.classification	Biology--Molecular	en_US
dc.subject.classification	Biochemistry	en_US
dc.title	Origin and diversification of tissue-resident fibroblasts	en_US
dc.type	doctoral thesis	en_US
thesis.degree.discipline	Medicine – Biochemistry and Molecular Biology	en_US
thesis.degree.grantor	University of Calgary	en_US
thesis.degree.name	Doctor of Philosophy (PhD)	en_US
ucalgary.item.requestcopy	true	en_US