Investigation of Depolarization as the Trigger for Lubricin and Hyaluronan Secretion from Non-Excitable Human Synovial Fibroblasts

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atmire.migration.oldid5734
dc.contributor.advisorGiles, Wayne
dc.contributor.advisorSchmidt, Tannin
dc.contributor.authorNarsingani, Karim
dc.contributor.committeememberBenseler, Susanne
dc.contributor.committeememberBraun, Andrew
dc.date.accessioned2017-07-04T22:07:49Z
dc.date.available2017-07-04T22:07:49Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractHyaluronan (HA) and Lubricin are major components of synovial fluid that are produced by synovial fibroblasts. Their secretion pathway remains unclear. However, calcium has been shown to play a major role. Depolarization may increase intracellular calcium. This study hypothesized that depolarization may lead to an increase in HA and Lubricin. Human synovial fibroblasts (HSF) from two donors were depolarized with 100mM extracellular K+. The media was analyzed for HA and Lubricin using and HA ELISA and AlphaLISA, respectively. Calcium fluorescence determined intracellular calcium concentration ([Ca2+]i). A generalized estimating equation (GEE) was used for statistical analysis. In the high K+ group HA concentration decreased (p=0.000); however, the Lubricin concentration did not change (p=1.000) and [Ca2+]i decreased. Depolarization with 100mM K+ for 12h did not increase [Ca2+]i, Lubricin levels and decreased HA levels in the HSF conditioned media. Results may indicate K+ is not the ion responsible for the resting membrane potential.en_US
dc.identifier.citationNarsingani, K. (2017). Investigation of Depolarization as the Trigger for Lubricin and Hyaluronan Secretion from Non-Excitable Human Synovial Fibroblasts (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26653en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26653
dc.identifier.urihttp://hdl.handle.net/11023/3922
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectPharmacology
dc.subject.otherSynoviocytes
dc.subject.otherLubricin
dc.subject.otherHyaluronan
dc.subject.otherElectrophysiology
dc.subject.otherFibroblasts
dc.titleInvestigation of Depolarization as the Trigger for Lubricin and Hyaluronan Secretion from Non-Excitable Human Synovial Fibroblasts
dc.typemaster thesis
thesis.degree.disciplineCardiovascular & Respiratory Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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