Regulating p21 Expression to Increase Chondrogenic Potential in Human Mesenchymal Progenitor Cells

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atmire.migration.oldid4950
dc.contributor.advisorKrawetz, Roman
dc.contributor.authorBertram, Karri
dc.contributor.committeememberRinker, Kristina
dc.contributor.committeememberBiernaskie, Jeff
dc.date.accessioned2016-10-04T19:05:04Z
dc.date.available2016-10-04T19:05:04Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractCartilage does not regenerate in humans, and therefore cartilage degeneration is a problem that affects a significant percentage of the population, including those with diseases such as Osteoarthritis (OA). The p21 knockout (p21-/-) mouse contains the only known single mutation in mammals that can induce a cartilage regenerative phenotype. Work in this thesis aims to identify p21 expression inhibitors for use in humans and to characterize their effects on human synovial mesenchymal progenitor cells (MPCs) during culture and chondrogenesis. I have identified one putative p21 expression inhibitor (acting through HSP90), that induces human synovial MPCs to display phenotypic properties similar to fibroblasts from p21-/- mice. Additionally, this inhibitor promotes cartilage formation in a mouse cartilage injury model. These results indicate that p21 inhibition through HSP90 may be a potential pharmaceutical target for stimulating chondrogenic regeneration for the treatment of cartilage defects or in cartilage degenerating diseases such as OA.en_US
dc.identifier.citationBertram, K. (2016). Regulating p21 Expression to Increase Chondrogenic Potential in Human Mesenchymal Progenitor Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27580en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27580
dc.identifier.urihttp://hdl.handle.net/11023/3397
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEngineering--Biomedical
dc.subject.classificationOsteoarthritisen_US
dc.subject.classificationp21en_US
dc.subject.classificationmesenchymal progenitor cellsen_US
dc.titleRegulating p21 Expression to Increase Chondrogenic Potential in Human Mesenchymal Progenitor Cells
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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