Antimicrobial Resistant Escherichia coli in Alberta's Rural Well Water

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atmire.migration.oldid5758
dc.contributor.advisorCheckley, Sylvia
dc.contributor.authorMeyer, Kelsey
dc.contributor.committeememberDeVinney, Rebekah
dc.contributor.committeememberLiljebjelke, Karen
dc.date.accessioned2017-07-20T15:26:17Z
dc.date.available2017-07-20T15:26:17Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractThe consumption of rural well water (RWW) contaminated with antimicrobial resistant (AMR) Escherichia coli has been linked to human carriage of resistance. Our objective was to determine whether AMR and extended spectrum beta lactamase (ESBL) producing E.coli are present in Alberta’s RWW. Resistant isolates were detected with an agar screen on (up to) 20 isolates from each sample, and AMR was measured with NARMS Sensititre(TM) panels. Disk diffusion assays detected ESBL-producing E.coli, and spatial clusters of AMR E.coli were assessed using ArcGIS (version 10.4.1) and SaTScan(TM) (version 9.4.4). Among 1129 samples, 22% contained AMR E.coli including four ESBL-producers. Resistance to three or more classes of antimicrobials was observed in 48% of AMR E.coli isolates, and a significant cluster of AMR E.coli was detected between Calgary and Lethbridge (p<0.05). Our results suggest AMR and ESBL-producing E.coli are present in Alberta’s rural well water, posing a risk to human and animal health.en_US
dc.identifier.citationMeyer, K. (2017). Antimicrobial Resistant Escherichia coli in Alberta's Rural Well Water (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/24938en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/24938
dc.identifier.urihttp://hdl.handle.net/11023/3976
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectVeterinary Science
dc.subjectBiophysics--Medical
dc.subjectEnvironmental Sciences
dc.subjectEpidemiology
dc.subjectPublic Health
dc.subject.otherE.coli
dc.subject.otherEscherichia coli
dc.subject.otherWater
dc.subject.otherDrinking water
dc.subject.othergroundwater
dc.subject.otherWell water
dc.subject.otherAntibiotic resistance
dc.subject.otherAntimicrobial resistance
dc.subject.otherESBL
dc.subject.otherExtended spectrum beta lactamase
dc.subject.otherAlberta
dc.subject.otherSTEC
dc.subject.otherShiga toxin producing E.coli
dc.titleAntimicrobial Resistant Escherichia coli in Alberta's Rural Well Water
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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