Functional Genomic Analysis of Novel Cell Cycle Transcription Factors in Schizosaccharomyce Pombe

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atmire.migration.oldid1032
dc.contributor.advisorChua, Gordon
dc.contributor.authorWood, Justin
dc.date.accessioned2013-05-30T16:00:40Z
dc.date.available2013-11-12T08:00:12Z
dc.date.issued2013-05-30
dc.date.submitted2013en
dc.description.abstractThe transcriptional-regulatory network in Schizosaccharomyces pombe remains poorly characterized with approximately 10% of transcription factors being well characterized. Attempts to complete the mapping of the transcriptional-regulatory network have been hindered by the observation that almost 90% of transcription factor deletions do not exhibit growth defects in rich media. To circumvent this issue, we have overexpressed six HA-tagged transcription factors (SPAC25B8.19, SPCC1393.08, phx1+, toe2+, toe3+, and toe4+) under the control of the nmt41 promoter. In order to identify the direct targets of these transcription factors, we performed expression microarray profiling, chromatin immunoprecipitation chip, qPCR, phenotypic replication, and genetic rescue on these strains. Gene ontology searching and motif finding to identify functional enrichment and conserved regulatory motifs were also performed. Through these techniques, we were able to describe putative roles for involvement of five transcription factors in the regulation of retrotransposable elements, meiosis, long term survival, septation, nuclear segregation, and cellular proliferation.en_US
dc.identifier.citationWood, J. (2013). Functional Genomic Analysis of Novel Cell Cycle Transcription Factors in Schizosaccharomyce Pombe (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26243en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26243
dc.identifier.urihttp://hdl.handle.net/11023/744
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGenetics
dc.subjectBiology--Molecular
dc.subjectPhysics--Molecular
dc.subject.classificationTranscription Factoren_US
dc.subject.classificationmicroarrayen_US
dc.subject.classificationFission Yeasten_US
dc.titleFunctional Genomic Analysis of Novel Cell Cycle Transcription Factors in Schizosaccharomyce Pombe
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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