The Effects Of Increased Stimulation On Sarcomere Number

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atmire.migration.oldid5108
dc.contributor.advisorHerzog, Walter
dc.contributor.authorEngel, Maria
dc.contributor.committeememberJoumaa, Venus
dc.contributor.committeememberSyme, Douglas
dc.contributor.committeememberSchmidt, Tannin
dc.contributor.committeememberButterfield, Timothy
dc.date.accessioned2016-11-02T21:31:30Z
dc.date.available2016-11-02T21:31:30Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractTabary et al. (1981) accelerated serial sarcomere loss by applying electrical stimulation, resulting in a 25% decrease in sarcomere number (SN) in the guinea pig soleus muscle within 12 hours. A duplication of these results was attempted in our laboratory. Rabbit lower hind limb muscles were stimulated for 10 hours continuously. As a result, muscles lost approximately 25% of sarcomeres in series. During laser diffraction analysis, it was observed that the first order diffraction band was wider in experimental compared to control muscles, implying some form of non-uniformity within the fascicle. Therefore, sarcomere length non-uniformity and structural disorganization was analyzed using light microscopy. Electrical stimulation indeed caused an increase in SL nonuniformity. In an attempt to examine the possibility of sarcomere loss in a more clinically relevant environment, muscle morphology and SN was analyzed in the genetically spastic mouse. The gastrocnemius muscle was shorter, but SN was generally unchanged.en_US
dc.identifier.citationEngel, M. (2016). The Effects Of Increased Stimulation On Sarcomere Number (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28108en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28108
dc.identifier.urihttp://hdl.handle.net/11023/3450
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectAnimal Physiology
dc.subjectBiology--Cell
dc.subjectPhysiology
dc.subject.classificationSkeletal Muscleen_US
dc.subject.classificationSerial Sarcomere Numberen_US
dc.subject.classificationFascicle Lengthen_US
dc.subject.classificationSarcomere Lengthen_US
dc.subject.classificationLaser Diffractionen_US
dc.subject.classificationCerebral Palsyen_US
dc.subject.classificationMuscle Damageen_US
dc.titleThe Effects Of Increased Stimulation On Sarcomere Number
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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