Expression and Role of Proteoglycan 4/Lubricin in the Human Pericardium: Implications as a Therapeutic to Prevent Post-Operative Pericardial Adhesions

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atmire.migration.oldid5749
dc.contributor.advisorFedak, Paul
dc.contributor.authorPark, Daniel
dc.date.accessioned2017-07-06T15:13:11Z
dc.date.available2017-07-06T15:13:11Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractRetrosternal pericardial adhesions form post-cardiac surgery and are associated with greater mortality risks for patients undergoing re-sternotomies. Fibrous adhesion development is mediated by cardiac myofibroblasts. Expression of proteoglycan 4 (PRG4) in the human pericardial anatomy and the biological functions of recombinant human PRG4 on human cardiac myofibroblasts was investigated. Expression of PRG4 in the human pericardial fluid and localized expression of PRG4 by pericardial mesothelial cells lining the pericardial cavity was observed. Recombinant human PRG4 prevented human cardiac myofibroblast adhesion to coated surfaces, attenuated transforming growth factor-β1 mediated collagen matrix gel contraction, decreased collagen fiber alignment by activated cardiac myofibroblasts and increased cell roundness. Replacing the lost endogenous PRG4 found in pericardial fluid with recombinant human PRG4 could attenuate the formation of post-operative pericardial adhesions and increase the safety and feasibility of re-sternotomies.en_US
dc.identifier.citationPark, D. (2017). Expression and Role of Proteoglycan 4/Lubricin in the Human Pericardium: Implications as a Therapeutic to Prevent Post-Operative Pericardial Adhesions (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26131en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26131
dc.identifier.urihttp://hdl.handle.net/11023/3931
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMedicine and Surgery
dc.subject.otherPericardium
dc.subject.otherCardiac Myofibroblast
dc.subject.otherProteoglycan 4
dc.titleExpression and Role of Proteoglycan 4/Lubricin in the Human Pericardium: Implications as a Therapeutic to Prevent Post-Operative Pericardial Adhesions
dc.typemaster thesis
thesis.degree.disciplineCardiovascular & Respiratory Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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