Tissue-Specific Regulation of Contraction and Elongation in the C. elegans Early Embryo

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dc.contributor.advisorMains, Paul E.
dc.contributor.authorDrewnik, Elizabeth Daisy
dc.contributor.committeememberMcGhee, James D.
dc.contributor.committeememberBrook, William J.
dc.contributor.committeememberCobb, John A.
dc.date2018-11
dc.date.accessioned2018-08-27T14:39:17Z
dc.date.available2018-08-27T14:39:17Z
dc.date.issued2018-08-20
dc.description.abstractMorphogenesis, a key feature of embryonic development, is driven by actin cytoskeleton contraction. Actin and myosin activity promote elongation of the Caenorhabditis elegans embryo from a ball of cells to a tubular worm. I used mutants in the elongation/contraction pathway to assess tissue-specific requirements of components regulating the contractile pathway. Contraction occurs mainly in the lateral embryonic epidermal cells, while the dorsoventral epidermal cells play a more passive role. Elongation is regulated by Rho kinase/LET-502, p-21 activating kinase/PAK-1, and myosin phosphatase/MEL-11. Though it is known that LET-502 and MEL-11 are expressed throughout the epidermis, in which cells the genes are necessary or sufficient is unknown. I used transgenic animals and body length measurements to show that LET-502 is sufficient in lateral but not dorsoventral cells, and that PAK-1 and MEL-11 are not sufficient in either lateral or dorsovetnral cells.en_US
dc.identifier.citationDrewnik, E. D. (2018). Tissue-Specific Regulation of Contraction and Elongation in the C. elegans Early Embryo (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/32834en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/32834
dc.identifier.urihttp://hdl.handle.net/1880/107654
dc.language.isoeng
dc.publisher.facultyCumming School of Medicine
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMorphogenesis
dc.subjectRho kinase
dc.subjectmyosin phosphatase
dc.subjectp21-activated kinase
dc.subjectC. elegans
dc.subjectcytoskeleton
dc.subjectactin
dc.subject.classificationBiologyen_US
dc.subject.classificationGeneticsen_US
dc.subject.classificationBiology--Molecularen_US
dc.subject.classificationPsychology--Developmentalen_US
dc.titleTissue-Specific Regulation of Contraction and Elongation in the C. elegans Early Embryo
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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