Co-infection with HRV and Pseudomonas aeruginosa Modulates Beta-Defensin-2 Expression from the Airway Epithelium

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atmire.migration.oldid3937
dc.contributor.advisorLeigh, Richard
dc.contributor.advisorProud, David
dc.contributor.authorArnason, Jason
dc.contributor.committeememberArmstrong, Glen
dc.contributor.committeememberChadee, Kris
dc.date.accessioned2015-12-21T23:07:39Z
dc.date.available2015-12-21T23:07:39Z
dc.date.issued2015-12-21
dc.date.submitted2015en
dc.description.abstractRespiratory infections are the most common triggers of acute exacerbations of chronic obstructive pulmonary disease (COPD), with human rhinovirus (HRV) and Pseudomonas aeruginosa being two common pathogens detected. Human beta-defensin (HBD)-2 is an antimicrobial peptide that plays an integral role in the innate defense response to infection. Here, we sought to determine how co-infection of airway cells with HRV and P. aeruginosa modulates HBD2 expression, and whether the normal HBD2 response to co-infections are altered in COPD subjects compared to healthy non-smokers. Synergistic increases in HBD2 from the airway epithelium were seen with the combination HRV and P. aeruginosa compared to either treatment alone. This synergistic response was dependent on flagellin acting through the TLR5 pathway. Finally, a reduction in HBD2 was observed in epithelial cells obtained from patients with COPD compared to cells from non-smokers following HRV and P. aeruginosa co-infection.en_US
dc.identifier.citationArnason, J. (2015). Co-infection with HRV and Pseudomonas aeruginosa Modulates Beta-Defensin-2 Expression from the Airway Epithelium (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25350en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25350
dc.identifier.urihttp://hdl.handle.net/11023/2691
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectImmunology
dc.titleCo-infection with HRV and Pseudomonas aeruginosa Modulates Beta-Defensin-2 Expression from the Airway Epithelium
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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