Identification of genes and interactions involved in syntrophic biodegradation under methanogenic conditions

Goldsmith, Ciara Ayame

Identification of genes and interactions involved in syntrophic biodegradation under methanogenic conditions

dc.contributor.advisor	Gieg, Lisa
dc.contributor.author	Goldsmith, Ciara Ayame
dc.contributor.committeemember	Hubert, Casey
dc.contributor.committeemember	Chua, Gordon
dc.date	2023-11
dc.date.accessioned	2023-07-18T20:47:54Z
dc.date.available	2023-07-18T20:47:54Z
dc.date.issued	2023-07
dc.description.abstract	Biodegradation, or the breakdown of pollutants by microorganisms, occurs in many global environments. Hydrocarbon biodegradation can be carried out by a diverse range of microorganisms, such as sulfate reducing microorganisms (SRM), and methanogens. Syntrophy, or the partnership of two species to metabolize a substrate despite an energetic barrier, is a dominant metabolic process in many environments where hydrocarbon biodegradation occurs. Understanding the syntrophic interactions that occur within microbial communities is important, since there is a possibility of uncovering novel microbial mechanisms and devise potential industrial applications. There is still much to be discovered about the specific interactions that occur within syntrophic partnerships, which is what this thesis project aimed to investigate. Metatranscriptomics was used to determine how gene expression changed when a bacterium maintained a syntrophic lifestyle, compared to an independent lifestyle in a pure culture. Co-cultures of the marine SRM Desulfatibacillum alkenivorans strain AK01 and either the methanogen Methanospirillum hungatei strain JF-1, or the marine methanogen Methanococcus maripaludis strain S2 were established. The differential gene expression analysis revealed that certain genes believed to be associated with syntrophic metabolic pathways and energy conservation were upregulated in both lactate-amended co-cultures. An additional contaminant organism within the cultures, Anaerovirgula multivorans, also may act as a syntroph based on gene expression and chemical activity data. Additionally, a co-occurrence network analysis was performed on eight mixed methanogenic hydrocarbon degrading consortia amended with different substrates. Multiple networks containing potential syntrophic organisms and methanogens were present, which could be used to establish future co-culture experiments.
dc.identifier.citation	Goldsmith, C. A. (2023). Identification of genes and interactions involved in syntrophic biodegradation under methanogenic conditions (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.uri	https://hdl.handle.net/1880/116760
dc.identifier.uri	https://dx.doi.org/10.11575/PRISM/41602
dc.language.iso	en
dc.publisher.faculty	Graduate Studies
dc.publisher.institution	University of Calgary
dc.rights	University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject	hydrocarbon biodegradation
dc.subject	syntrophy
dc.subject	methanogenesis
dc.subject	environmental microbiology
dc.subject	petroleum microbiology
dc.subject	metatranscriptomics
dc.subject	metagenomics
dc.subject.classification	Microbiology
dc.subject.classification	Energy
dc.subject.classification	Environmental Sciences
dc.subject.classification	Biology--Molecular
dc.title	Identification of genes and interactions involved in syntrophic biodegradation under methanogenic conditions
dc.type	master thesis
thesis.degree.discipline	Biological Sciences
thesis.degree.grantor	University of Calgary
thesis.degree.name	Master of Science (MSc)
ucalgary.thesis.accesssetbystudent	I do not require a thesis withhold – my thesis will have open access and can be viewed and downloaded publicly as soon as possible.