Characterization of Antimicrobial and Metal Resistance in Contemporary and Historical clinical isolates of Histophilus somni from Feedlot cattle in Alberta

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atmire.migration.oldid4976
dc.contributor.advisorLiljebjelke, Karen
dc.contributor.authorBhatt, Krishna
dc.contributor.committeememberWindeyer, Claire
dc.contributor.committeememberTimsit, Edouard
dc.contributor.committeememberStorey, Douglas
dc.date.accessioned2016-10-04T15:57:11Z
dc.date.available2016-10-04T15:57:11Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractThis study was conducted to determine antimicrobial and metal resistance, associated resistant genes, and mobile genetic elements in contemporary (2012-2016, n=63) and historical (1980’s, n=31) clinical isolates of Histophilus somni from feedlot cattle in Alberta. A significant increase in antimicrobial resistance was observed in the contemporary isolates compared to the historical (P<0.001). Sixty-two percent of the contemporary isolates exhibited multidrug resistance (resistance to 3 drug classes). Sixty-eight percent and 52% of the contemporary isolates showed tolerance to copper and zinc concentrations above one millimolar, respectively. Multicopper oxidase and cation efflux pump genes associated with copper and zinc tolerance and tet(H) gene associated with oxytetracycline resistance were identified. We identified integrative conjugative element carrying antimicrobial, copper, and zinc resistance genes, which indicates the possibility of co-selection of antimicrobial resistance due to copper and zinc selection pressure, and the dissemination of resistance by horizontal gene transfer.en_US
dc.identifier.citationBhatt, K. (2016). Characterization of Antimicrobial and Metal Resistance in Contemporary and Historical clinical isolates of Histophilus somni from Feedlot cattle in Alberta (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25268en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25268
dc.identifier.urihttp://hdl.handle.net/11023/3377
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectBiology--Molecular
dc.subjectVeterinary Science
dc.subject.classificationantimicrobial resistanceen_US
dc.subject.classificationHistophilus somnien_US
dc.subject.classificationfeedlot cattleen_US
dc.subject.classificationcopperen_US
dc.subject.classificationzincen_US
dc.subject.classificationmetal toleranceen_US
dc.titleCharacterization of Antimicrobial and Metal Resistance in Contemporary and Historical clinical isolates of Histophilus somni from Feedlot cattle in Alberta
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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