Sarcomere length measurement reliability in single myofibrils

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dc.contributor.authorSchmidt, Jonas
dc.contributor.authorJinha, Azim
dc.contributor.authorHerzog, Walter
dc.date.accessioned2021-07-15T19:56:05Z
dc.date.available2021-07-15T19:56:05Z
dc.date.issued2021-07-12
dc.description.abstractSarcomere length non-uniformities occur at all structural levels of skeletal muscles and have been associated with important mechanical properties. Changes in sarcomere length non-uniformities in the nano- and sub-nanometer range have been used to explain muscle properties and contractile mechanisms. Typically, these measurements rely on light microscopy with a limited spatial resolution. One critical aspect in sarcomere length determination is the relatively arbitrary choice of intensity thresholds used to delineate sarcomere structures, such as A-bands or Z-lines. In experiments, these structures are typically distorted, intensity profiles vary, and baselines drift, resulting in asymmetric intensity patterns, causing changes in the centroid location of these structures depending on threshold choice, resulting in changes of sarcomere lengths. The purpose of this study was to determine the changes in (half-) sarcomere lengths associated with small changes in the A-band threshold choice. Sarcomere and half-sarcomere length changes for minute variations in A-band threshold were 28 nm (± 28 nm) and 18 nm (± 22 nm), respectively, and for the entire feasible range of thresholds across A-bands were 123 nm (± 88 nm) and 99 nm (± 105 nm), respectively. We conclude from these results that (half-) sarcomere lengths in the nanometer range obtained with light microcopy are noise, and the functional implications associated with such data should be discarded. We suggest that a functional resolution for sarcomere length of 100 nm (0.1 µm) is reasonable and 50 nm (0.05 µm) might be possible under ideal conditions.en_US
dc.identifier.citationSchmidt, J., Jinha, A., & Herzog, W. (2021). Sarcomere length measurement reliability in single myofibrils. Journal of Biomechanics, 126, 110628. doi:10.1016/j.jbiomech.2021.110628en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jbiomech.2021.110628en_US
dc.identifier.urihttp://hdl.handle.net/1880/113627
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.publisher.departmentHuman Performance Laben_US
dc.publisher.facultyKinesiologyen_US
dc.publisher.hasversionacceptedVersionen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.institutionUniversity of Bremen, Bremen, Germanyen_US
dc.publisher.institutionFederal University of Santa Catarina, Florianopolis, SC, Brazilen_US
dc.publisher.policyhttps://www.elsevier.com/about/policies/sharingen_US
dc.rightsUnless otherwise indicated, this material is protected by copyright and has been made available with authorization from the copyright owner. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0en_US
dc.subjectMuscleen_US
dc.subjectSarcomere Lengthen_US
dc.subjectNon-Uniformityen_US
dc.subjectMyofibrilen_US
dc.subjectMeasurementsen_US
dc.subjectTitinen_US
dc.subjectResidual force enhancementen_US
dc.subjectStabilityen_US
dc.subjectResidual force depressionen_US
dc.subjectForce-length relationshipen_US
dc.titleSarcomere length measurement reliability in single myofibrilsen_US
dc.typejournal articleen_US
ucalgary.item.requestcopyfalseen_US
ucalgary.scholar.levelFacultyen_US
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