Shear Stress Modulates Gene Expression in Normal Human Dermal Fibroblasts

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atmire.migration.oldid5323
dc.contributor.advisorRinker, Kristina
dc.contributor.authorZabinyakov, Nikita
dc.contributor.committeememberRinker, Kristina
dc.contributor.committeememberXilong, Zheng
dc.contributor.committeememberKrawetz, Roman
dc.contributor.committeememberRancourt, Derrick
dc.contributor.committeememberBertram, John
dc.date.accessioned2017-02-02T18:12:23Z
dc.date.available2017-02-02T18:12:23Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractApplied mechanical forces, such as those resulting from fluid flow, trigger cells to change their functional behavior or phenotype. However, there is little known about how fluid flow affects fibroblasts. The hypothesis of this thesis is that dermal fibroblasts undergo significant changes of expression of differentiation genes after exposure to fluid flow (or shear stress). To test the hypothesis, human dermal fibroblasts were exposed to laminar steady fluid flow for 20 and 40 hours and RNA was collected for microarray analysis. Gene expression data was processed using gene network analysis, pathway analysis, and gene functional analysis with comparison to data from publicly available data sets. Additional treatment with PI3K/mTOR pathway inhibitor, PI-103, was performed to evaluate pathway involvement in flow modulation of gene expression. Results from overall transcription analysis demonstrated that fluid flow modulated many genes in fibroblasts including those related to differentiation, development and TGF-β pathway regulation.en_US
dc.identifier.citationZabinyakov, N. (2017). Shear Stress Modulates Gene Expression in Normal Human Dermal Fibroblasts (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27775en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27775
dc.identifier.urihttp://hdl.handle.net/11023/3639
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEngineering--Biomedical
dc.subject.othershear stress
dc.subject.otherFibroblasts
dc.subject.otherflow chamber
dc.subject.othermicroarray
dc.subject.otherpluripotency
dc.subject.othertrans-differentiation
dc.titleShear Stress Modulates Gene Expression in Normal Human Dermal Fibroblasts
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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