Development of vascular regulation in the zebrafish embryo.

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dc.contributor.advisorChilds, Sarah J.
dc.contributor.authorBahrami, Nabila
dc.contributor.committeememberCole, William C.
dc.contributor.committeememberGordon, Grant Robert J.
dc.date2019-11
dc.date.accessioned2019-09-23T20:10:36Z
dc.date.available2019-09-23T20:10:36Z
dc.date.issued2019-09-19
dc.description.abstractThe vascular system is placed under enormous stress at the onset of cardiac contractility and blood flow. Nascent blood vessel tubes initially consist of a thin endothelial wall and rapidly acquire support from mural cells (pericytes and vascular smooth muscle cells; vSMCs). Following their association with vessels, mural cells acquire vasoactive ability (contraction and relaxation). However, we have little information as to when this vasoactivity first develops, and the extent to which each mural cell type contributes to vascular tone regulation during development. For the first time in an in vivo system, we highlight the dynamic changes in mural cell vasoactivity during development. We assess mural cell vasoactivity in the early zebrafish (Danio rerio) cerebral vasculature in response to pharmacological agents. We determine that pericyte-covered vessels constrict and dilate at 4 days post fertilization (dpf) but not at 6 dpf. The prostaglandin EP4 receptor contributes to pericyte-covered vessel dilation at 4 dpf. In contrast, vSMC-covered vessels constrict but do not dilate at 4 dpf. At 6 dpf, vSMC-covered vessels continue to constrict but only dilate from a pre-constricted state. Using genetic ablation, we demonstrate that mural cell contraction and relaxation is an active response by pericytes and vSMCs. Thus, we show that both pericytes and vSMCs have the ability to regulate cerebral vascular tone but at different stages of development. Pericytes are involved in regulating vessel diameters prior to the maturation of the vSMCs. Once vSMCs mature, pericytes are no longer active, and only vSMCs mediate vasomotor activity in the developing embryonic brain of zebrafish. The onset of vSMC vasoactivity corresponds to the development of increased neuronal activity and neurovascular coupling.en_US
dc.identifier.citationBahrami, N. (2019). Development of vascular regulation in the zebrafish embryo. (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/37105
dc.identifier.urihttp://hdl.handle.net/1880/111043
dc.language.isoengen_US
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectpericyteen_US
dc.subjectvascular smooth muscle cellen_US
dc.subjectcerebral vasculatureen_US
dc.subjectdilationen_US
dc.subjectconstrictionen_US
dc.subjectcontractionen_US
dc.subjectrelaxationen_US
dc.subjectmicroscopyen_US
dc.subjectvasoactive agentsen_US
dc.subjectvascular toneen_US
dc.subjectvasoactivityen_US
dc.subjectmural cellsen_US
dc.subjectgenetic ablationen_US
dc.subjectnitric oxide (NO)en_US
dc.subjectadrenergicen_US
dc.subjectzebrafishen_US
dc.subject.classificationBiology--Molecularen_US
dc.subject.classificationBiochemistryen_US
dc.titleDevelopment of vascular regulation in the zebrafish embryo.en_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Biochemistry and Molecular Biologyen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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