Exploring Protein O-GlcNAcylation as a Novel Diagnostic Tool for Type II Mellitus and the Impact of Exercise on Cardiac Protein O-GlcNAcylation

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atmire.migration.oldid2165
dc.contributor.advisorShearer, Jane
dc.contributor.advisorBelke, Darrell
dc.contributor.authorMyslicki, Jason
dc.date.accessioned2014-05-16T16:54:44Z
dc.date.available2014-06-16T07:00:44Z
dc.date.issued2014-05-16
dc.date.submitted2014en
dc.description.abstractThe purpose of this study was to explore the sensitivity of O-linked β-N-acetylglucosamine (O-GlcNAc) in relation to various anthropometric and metabolic markers in healthy young adults and then to investigate how a chronic exercise regime affects this important post-translation signalling pathway in a Type II diabetic (T2DM) mouse model. Whole blood was collected from clinically healthy young adult males (n=24) and cardiac tissue was collected from diabetic (db/db) and control (db+) mice (n=9-10/group) that underwent six weeks of exercise or were designated sedentary. Western blot analysis was performed on all samples. Human whole blood exhibited greater sensitivity to Homeostatic Model of Assessment – Insulin Resistance (HOMA-IR) than Hemoglobin A1c (HbA1c) demonstrating its potential utility as a screening tool with strong diagnostic value. Six weeks of running wheel exercise did not alter protein O-GlcNAcylation in db/db or db+ mice. Protein O-GlcNAcylation is a valuable tool in assessing the efficacy of exercise in preventing T2DM.en_US
dc.identifier.citationMyslicki, J. (2014). Exploring Protein O-GlcNAcylation as a Novel Diagnostic Tool for Type II Mellitus and the Impact of Exercise on Cardiac Protein O-GlcNAcylation (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26098en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26098
dc.identifier.urihttp://hdl.handle.net/11023/1526
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyKinesiology
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectPhysiology
dc.subject.classificationO-GlcNAcen_US
dc.subject.classificationType II Diabetes Mellitusen_US
dc.subject.classificationInsulin Resistanceen_US
dc.subject.classificationExerciseen_US
dc.subject.classificationpost translational protein modificationen_US
dc.titleExploring Protein O-GlcNAcylation as a Novel Diagnostic Tool for Type II Mellitus and the Impact of Exercise on Cardiac Protein O-GlcNAcylation
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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