The In Vitro Development of a Metabolomic Test for Response to Antineoplastic Drugs

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atmire.migration.oldid5284
dc.contributor.advisorBathe, Dr. Oliver
dc.contributor.authorAmin, Shahil
dc.contributor.committeememberKopciuk, Dr. Karen
dc.contributor.committeememberBebb, Dr. Gwyn
dc.contributor.committeememberVogel, Dr. Hans J.
dc.date.accessioned2017-01-30T22:45:22Z
dc.date.available2017-01-30T22:45:22Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractBackground: Only a fraction of patients benefit from any given chemotherapeutic. An assay that enables early identification of individuals who are/are not benefiting from a drug would be useful. We postulate that a response to systemic therapy is associated with characteristic changes in extracellular metabolites. Methods: Multiple tumor cell lines were exposed to a variety of chemotherapeutics. Supernatants collected at baseline and at 72 hours were analyzed by gas chromatography-mass spectrometry to identify metabolomic changes. An MTT assay was used to quantify growth inhibition. Results: We identified 23 candidate metabolites that change with response to therapy, independent of cell and drug type. This model had a sensitivity and specificity of 0.714 and 0.813 respectively in an ROC analysis. Conclusions: The candidate metabolites identified will be assessed in clinical samples from patients treated with systemic therapy. Identification of response-related changes in the circulating metabolome may represent a novel means of detecting response to chemotherapy.en_US
dc.identifier.citationAmin, S. (2017). The In Vitro Development of a Metabolomic Test for Response to Antineoplastic Drugs (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28224en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28224
dc.identifier.urihttp://hdl.handle.net/11023/3603
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMedicine and Surgery
dc.subjectOncology
dc.subject.otherResponse Biomarker
dc.subject.otherMetabolomics
dc.subject.otherCancer
dc.subject.otherRECIST
dc.subject.otherAssessing Response
dc.subject.otherGas Chromatography Mass Spectrometry
dc.subject.otherAdaptive Biomarker
dc.subject.otherSystemic Therapy
dc.subject.otherAntineoplastic Agents
dc.titleThe In Vitro Development of a Metabolomic Test for Response to Antineoplastic Drugs
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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