Development of an In Vitro Microelectric Cell Sensing Technique to Assess Cytotoxicity of Ocular Irritants

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atmire.migration.oldid4041
dc.contributor.advisorKinniburgh, David W
dc.contributor.advisorSlater, Donna M.
dc.contributor.authorBerezowski, Kathryn L
dc.contributor.committeememberHuang, Dorothy Y
dc.contributor.committeememberGabos, Stephan
dc.date.accessioned2016-01-22T22:04:16Z
dc.date.available2016-01-22T22:04:16Z
dc.date.issued2016-01-22
dc.date.submitted2016en
dc.description.abstractIn this study, a novel alternative in vitro method for assessing ocular irritancy was developed. This was completed by evaluating the cytotoxicity of nine compounds exposed to a panel of three cell lines using a microelectric cell sensing technology, the xCELLigenceTM RTCA MP. With this novel method, it was successfully demonstrated that the cell lines used in the study were able to show different responses to irritants and non-irritants, but depending on the compounds tested and measures of cytotoxicity used, it was inconclusive if one particular cell line or measure was superior for ranking the test compounds. This study also served to elucidate the difficulties that surround the complex problem of developing alternative methods to the current gold standard in vivo Draize test.en_US
dc.identifier.citationBerezowski, K. L. (2016). Development of an In Vitro Microelectric Cell Sensing Technique to Assess Cytotoxicity of Ocular Irritants (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25668en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/25668
dc.identifier.urihttp://hdl.handle.net/11023/2768
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectToxicology
dc.subject.classificationToxicologyen_US
dc.subject.classificationAlternative Methoden_US
dc.subject.classificationIn Vitro methoden_US
dc.subject.classificationOcular Toxicologyen_US
dc.subject.classificationCytotoxicityen_US
dc.titleDevelopment of an In Vitro Microelectric Cell Sensing Technique to Assess Cytotoxicity of Ocular Irritants
dc.typemaster thesis
thesis.degree.disciplineMedical Science
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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