Vitamin D induces the release of IL-1b via activation of the NLRP3 inflammasome

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atmire.migration.oldid211
dc.contributor.advisorMacDonald, Justin
dc.contributor.advisorBeck, Paul
dc.contributor.authorTulk, Sarah
dc.date.accessioned2012-07-30T22:05:41Z
dc.date.available2015-07-31T07:00:49Z
dc.date.issued2012-07-30
dc.date.submitted2012en
dc.description.abstractThe Crohn’s disease pathogenesis has been proposed to involve a hypoactive innate immune response and impaired acute inflammatory response. Recently, vitamin D3 has been shown to induce expression of the innate immune receptor, NOD2. Herein, PMA-differentiated THP-1 cells were treated with the active form of vitamin D3, 1,25(OH)2D3, and assessed for release of the pro-inflammatory cytokine IL-1β. We present novel findings that 1,25(OH)2D3 induces the release of IL-1β from PMA-differentiated THP-1 cells. Further, circulating vitamin D3, 25(OH)D3, was also able to induce the IL-1β response. Both 1,25(OH)2D3-induced and 25(OH)D3-induced IL-1β release were found to be dependent on the NLRP3 inflammasome. These novel findings support a beneficial role for vitamin D3 in Crohn’s disease; by boosting basal IL-1β secretion from macrophages, vitamin D3 could help counteract the impaired innate immune and acute inflammatory responses seen in this disease.en_US
dc.description.embargotermsIndefinite
dc.identifier.citationTulk, S. (2012). Vitamin D induces the release of IL-1b via activation of the NLRP3 inflammasome (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28659en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28659
dc.identifier.urihttp://hdl.handle.net/11023/142
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyMedicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectImmunology
dc.subject.classificationGastrointestinalen_US
dc.subject.classificationmacrophageen_US
dc.subject.classificationInflammasomeen_US
dc.subject.classificationinterleukin 1 betaen_US
dc.subject.classificationVitamin Den_US
dc.titleVitamin D induces the release of IL-1b via activation of the NLRP3 inflammasome
dc.typemaster thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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