Muscular loading of joints triggers cellular secretion of PRG4 into the joint fluid

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dc.contributor.authorAbusara, Ziad
dc.contributor.authorKrawetz, Roman J.
dc.contributor.authorSteele, Bridgett L.
dc.contributor.authorDuVall, Michael M.
dc.contributor.authorSchmidt, Tannin A.
dc.contributor.authorHerzog, Walter
dc.date.accessioned2018-09-26T19:22:46Z
dc.date.available2018-09-26T19:22:46Z
dc.date.issued2013-04-26
dc.description.abstractWe developed a novel testing system that allows quantification of joint loading and permits analysis of changes in total protein and PRG4 contents in joint fluid of intact knees in live mice. A sequence of 15 repeat, isometric muscular contractions of "low" intensity (less than 50% of the maximal isometric muscular force), and "high" intensity (greater than 55% of maximal) were applied repeatedly (up to five times with a 15 min rest between contractions) to the mouse knee. Increases in knee joint loading were accompanied with significant increases in total protein (p<0.0001) and PRG4 concentrations in the synovial fluid. Total protein and PRG4 concentrations decreased with repeated "high" intensity loading. However, the addition of cell secretion inhibitors to the knee prior to muscular loading resulted in PRG4 levels that remained below the detection limit for all loading conditions. These results suggest that changes in synovial fluid proteins and PRG4 concentrations upon joint loading are mediated by cells within the joint, and that these changes may be used as quantitative indicators for the intensity and duration of acute joint loading, and might serve as a powerful clinical tool to assess the effectiveness of rehabilitation and prevention exercise programs.en_US
dc.description.grantingagencyCanadian Institutes of Health Research - Other Programsen_US
dc.identifier.citationAbusara, Z., Krawetz, R., Steele, B., DuVall, M., Schmidt, T., & Herzog, W. (2013). Muscular loading of joints triggers cellular secretion of PRG4 into the joint fluid. Journal of Biomechanics, 46(7), 1225–1230. https://doi.org/10.1016/j.jbiomech.2013.02.027en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jbiomech.2013.02.027en_US
dc.identifier.issn1873-2380
dc.identifier.urihttp://hdl.handle.net/1880/108060
dc.language.isoenen_US
dc.publisherJournal of Biomechanicsen_US
dc.publisher.departmentHuman Performance Laben_US
dc.publisher.facultyKinesiologyen_US
dc.publisher.hasversionPost-printen_US
dc.publisher.institutionUniversity of Calgaryen_US
dc.publisher.policyhttps://www.jbiomech.com/content/authorinfo?code=bm-siteen_US
dc.rightsUnless otherwise indicated, this material is protected by copyright and has been made available with authorization from the copyright owner. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.subjectmuscleen_US
dc.subjectmuscular loadingen_US
dc.subjectProteoglycan 4 (PRG4)en_US
dc.subjectcartilageen_US
dc.subjectsynovial fluiden_US
dc.subjectosteoarthritis (OA)en_US
dc.titleMuscular loading of joints triggers cellular secretion of PRG4 into the joint fluiden_US
dc.typejournal articleen_US
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