Comparing nanoparticle physical properties with bio-uptake

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dc.contributor.advisorCramb, David T.
dc.contributor.authorYaehne, Kristin Lee
dc.date.accessioned2017-12-18T22:30:17Z
dc.date.available2017-12-18T22:30:17Z
dc.date.issued2012
dc.descriptionBibliography: p. 115-127en
dc.descriptionSome pages are in colour.en
dc.description.abstractNanoparticle-organismal interactions depend on nanoparticle (NP) physical properties and tissue type. Angiogenic vasculature, characteristic of neoplastic diseases and developing tissues, has fenestrations between endothelial cells in which NPs can accumulate. This phenomenon is integral to nanomedicine and nanotoxicology, so we systemically injected dye-impregnated, polystyrene FluoSpheres (FS) into angiogenic chicken embryo chorioallantoic membrane (CAM) to determine bioaccumulation patterns and associated kinetics within the blood. FS concentration was monitored in the CAM using two-photon excitation fluorescence correlation spectroscopy and bio-uptake rate constants in flowing vessels were determined. Negatively charged, carboxylate-coated FS under 500 nm in diameter showed uptake in flowing vessels, while amine FS immediately agglomerated and adhered to the endothelium. Carboxylate FS uptake rate constants showed linear dependence on R-;/, indicating that NP surface area footprint determines uptake rates. A predictive bio-uptake model was developed based on NP physical properties, which clarifies the passive nature of NP uptake into angiogenic tissues.
dc.format.extentxv, 127 leaves : ill. ; 30 cm.en
dc.identifier.citationYaehne, K. L. (2012). Comparing nanoparticle physical properties with bio-uptake (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/4694en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/4694
dc.identifier.urihttp://hdl.handle.net/1880/105695
dc.language.isoeng
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.titleComparing nanoparticle physical properties with bio-uptake
dc.typemaster thesis
thesis.degree.disciplineChemistry
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
ucalgary.thesis.accessionTheses Collection 58.002:Box 2098 627942970
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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