gob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans

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dc.contributor.advisorMcGhee, James D.
dc.contributor.authorKormish, Jay Dene
dc.date.accessioned2005-08-10T19:22:48Z
dc.date.available2005-08-10T19:22:48Z
dc.date.issued2004
dc.descriptionBibliography: p. 194-217en
dc.descriptionSome pages are in colour.en
dc.description.abstractA genetic screen was developed with the intention of finding C. elegans genes that are required for intestine development or differentiation. The screen was based on the gut obstructed phenotype of the elt-2 null mutant. elt-2 is a GATA transcription factor believed to be the major transcription factor driving intestine differentiation. It was anticipated that screening for a similar gut obstructed mutant phenotype could identify genes that were functioning redundantly to elt-2 or downstream of elt-2. One of the mutations identified in this screen, gob-1(ca17), was mapped to the right-hand side of the X chromosome. Positional cloning of the gob-1(ca17) allele showed that it was a deletion of approximately 15 open reading frames. RNA-mediated interference to a gene in this region, H13N06.3, phenocopied the early larval arrest and gut obstructed phenotype of gob-1(ca17) and was thus identified as the gene responsible for this phenotype. The gob-1 RNAi mutant causes an early defect in the intestine. The gob-1 gene and GOB-1 protein are expressed specifically in the intestine during its early development. GOB-1 is a member of the Haloacid dehalogenase (HAD)-like hydrolase superfamily and is the first identified trehalose-6-phosphate phosphatase in the nematode phylum. GOB-1’s function in trehalose metabolism is currently unclear but mounting evidence suggests that GOB-1 may have a function required for viability of the worm independent of trehalose synthesis. ELT-2 is sufficient but not necessary for early embryonic expression of GOB-1. The ability of ELT-2 to drive gob-1 expression suggests that loss of gob-1 iv function may be a component of the later elt-2 loss of function defect in the larval intestine.en
dc.format.extent4604443 bytes
dc.format.extentxvii, 217 leaves : ill. ; 30 cm.en
dc.format.mimetypeapplication/pdf
dc.identifier.citationKormish, J. D. (2004). gob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans (Doctoral thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/11628en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/11628
dc.identifier.urihttp://hdl.handle.net/1880/41335
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsAttribution Non-Commercial No Derivatives 2.5*
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/*
dc.titlegob-1 is a trehalose-6-phosphate phosphatase required for intestine development in the nematode caenorhabditis elegans
dc.typedoctoral thesis
thesis.degree.disciplineBiochemistry and Molecular Biology
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameDoctor of Philosophy (PhD)
ucalgary.item.requestcopytrue
ucalgary.thesis.notesUARCen
ucalgary.thesis.uarcreleaseyen
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