Investigating the Effects of Probiotic Supplementation on the Gut-Brain Axis in Autism Spectrum Disorder

dc.contributor.advisorShearer, Jane
dc.contributor.authorPochakom, Angela
dc.contributor.committeememberPittman, Quentin
dc.contributor.committeememberReimer, Raylene
dc.date2022-11
dc.date.accessioned2022-07-11T19:54:03Z
dc.date.available2022-07-11T19:54:03Z
dc.date.issued2022-07
dc.description.abstractHistorically, the purpose of probiotic use in autism spectrum disorder (ASD) has been associated with alleviating co-morbid gastrointestinal symptoms. Recent studies have shown promise for the use of probiotics in modulating brain function to improve the behavioural symptoms of ASD through the microbiota-gut-brain axis. In the present study, we assessed the impact of two probiotic strains in mitigating autism-related symptomology in the BTBR T+ Itpr3tf/J mouse model of autism spectrum disorder (ASD). Male juvenile BTBR mice were randomized into 1) control, 2) Lr probiotic (1 x 109 CFU/mL Lacticaseibacillus rhamnosus HA-114), and 3) Ls probiotic groups (1 x 109 CFU/mL Ligilactobacillus salivarius HA-118) (n=18-21/group), receiving treatments in drinking water for 4-weeks. Gut microbiota profiling by 16S rRNA showed Lr, but not Ls supplementation, to increase microbial richness and phylogenetic diversity, with a rise in potential anti-inflammatory and butyrate-producing taxa. Assessing serum and brain metabolites, Lr and Ls supplementation produced distinct metabolic profiles, with Lr treatment elevating concentrations of potentially beneficial neuroactive compounds, such as 5-aminovaleric acid and choline. As mitochondrial dysfunction is often observed in ASD, we assessed mitochondrial oxygen consumption rates in the prefrontal cortex and hippocampus. No differences were observed for either treatment. Both Lr and Ls treatment reduced behavioural deficits in social novelty preference. However, no changes in hyperactivity, repetitive behaviour, and sociability were observed. Results show Lr to impart positive changes along the microbiota-gut-brain axis, exhibiting beneficial effects on selected behaviour, gut microbial diversity, and metabolism in BTBR mice.en_US
dc.identifier.citationPochakom, A. (2022). Investigating the effects of probiotic supplementation on the gut-brain axis in autism spectrum disorder (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/39893
dc.identifier.urihttp://hdl.handle.net/1880/114825
dc.publisher.facultyCumming School of Medicineen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subjectGut Microbiotaen_US
dc.subjectAutismen_US
dc.subjectBehaviouren_US
dc.subjectProbioticsen_US
dc.subjectMetabolomicsen_US
dc.subjectMitochondriaen_US
dc.subjectGut-Brain Axisen_US
dc.subject.classificationEducation--Sciencesen_US
dc.subject.classificationNeuroscienceen_US
dc.subject.classificationPsychology--Developmentalen_US
dc.titleInvestigating the Effects of Probiotic Supplementation on the Gut-Brain Axis in Autism Spectrum Disorderen_US
dc.typemaster thesisen_US
thesis.degree.disciplineMedicine – Medical Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrueen_US
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