PDGF Signaling in Sertoli Cell Cilia and Seminiferous Tubule Morphogenesis

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dc.contributor.advisorDobrinski, Ina
dc.contributor.authorAhmadi Jeyhoonabadi, Maryam
dc.contributor.committeememberGilch, Sabine
dc.contributor.committeememberJiami Guo
dc.date0023-11
dc.date.accessioned2023-07-20T16:35:23Z
dc.date.available2023-07-20T16:35:23Z
dc.date.issued2023-07
dc.description.abstractImmature porcine testicular Sertoli cells possess primary cilia, organelles that transmit extracellular signals into the cell. A yet unidentified ciliary signal in Sertoli cells has been found necessary for in vitro morphogenesis of tubules and testicular organoids. Herein, we hypothesized that platelet-derived growth factor receptor alpha (PDGFRα) signaling through the cilia of the Sertoli cells modifies cell migration and extracellular matrix (ECM) production, consequently altering in vitro formation of testicular organoids and seminiferous tubules. Immunocytochemistry revealed PDGFRα was present on Sertoli cell cilia as well as the rest of the cell membrane and cytoplasm. Inhibiting PDGFRα signaling did not prevent organoid and tubule formation. However, it appeared to correlate with ablation of cytoplasmic extensions from the surface of the in vitro formed tubules. Inhibition of PDGFRα signaling resulted in reduction in cell area, increase in the percentage of cells with prominent peripheral paxillin expression, and increase in the number of ciliated cells and cilia length. Immunohistochemistry revealed that cilia, which colocalized with PDGFRα, were positioned away from the basement membrane of the seminiferous tubules, close to the Sertoli cell nuclei, in an area that was occupied with Sertoli cell cytoplasm. When observed via transmission electron microscopy, Sertoli cell cilia exhibited ciliary pockets around their axoneme. These results demonstrate that PDGFRα activity in Sertoli cells can influence in vitro tubule morphogenesis, possibly by altering cell cytoskeleton, cell-ECM interaction, and cilia. However, the role of cilia-specific signaling remains to be determined.
dc.identifier.citationAhmadi Jeyhoonabadi, M. (2023). PDGF signaling in Sertoli cell cilia and seminiferous tubule morphogenesis (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.
dc.identifier.urihttps://hdl.handle.net/1880/116765
dc.identifier.urihttps://dx.doi.org/10.11575/PRISM/41607
dc.language.isoen
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgary
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subject.classificationVeterinary Science
dc.titlePDGF Signaling in Sertoli Cell Cilia and Seminiferous Tubule Morphogenesis
dc.typemaster thesis
thesis.degree.disciplineVeterinary Medical Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.thesis.accesssetbystudentI require a thesis withhold – I need to delay the release of my thesis due to a patent application, and other reasons outlined in the link above. I have/will need to submit a thesis withhold application.
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