Impact of Knee Joint Loading on Site-Specific Cartilage Gene-Expression in a Porcine Model

Simple item page
atmire.migration.oldid5196
dc.contributor.advisorLi, Leping
dc.contributor.authorOtoo, Baaba
dc.contributor.committeememberHerzog, Walter
dc.contributor.committeememberHart, David
dc.contributor.committeememberEdwards, Brent
dc.date.accessioned2017-01-02T19:45:42Z
dc.date.available2017-01-02T19:45:42Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractCyclic mechanical loading of cartilage induces stresses and fluid flow which are thought to modulate chondrocyte metabolism. The uneven surface plus the heterogeneity of cartilage within a joint makes stress and fluid pressure distribution in the tissue non-uniform, and gene expression may vary at different sites as a function of load magnitude, frequency and time. In previous studies, cartilage explants were used for loading tests to investigate cartilage biological response to mechanical loading, which did not reflect well the loading conditions in a joint. In contrast, we used loading tests of intact knee joints, providing a more physiologically relevant mechanical environment. Gene expression levels of loaded samples were compared with that of corresponding control samples to establish variations. Variations in gene expression levels after mechanical loading provide an indication of the effect of local topography and structure of the cartilage on the metabolic activity of the tissue regulated by mechanical loading.en_US
dc.identifier.citationOtoo, B. (2016). Impact of Knee Joint Loading on Site-Specific Cartilage Gene-Expression in a Porcine Model (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26460en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26460
dc.identifier.urihttp://hdl.handle.net/11023/3517
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGenetics
dc.subjectEngineering--Biomedical
dc.subjectEngineering--Mechanical
dc.subject.classificationBiomechanicsen_US
dc.subject.classificationCartilageen_US
dc.subject.classificationGene expressionen_US
dc.subject.classificationJoint loadingen_US
dc.subject.classificationRNA extractionen_US
dc.subject.classificationRT-qPCRen_US
dc.titleImpact of Knee Joint Loading on Site-Specific Cartilage Gene-Expression in a Porcine Model
dc.typemaster thesis
thesis.degree.disciplineMechanical and Manufacturing Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Collections
Open Theses and Dissertations