Force Based Comparison Between Naïve and Activated CD4+ T Cells during Interaction with Dendritic Cells

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atmire.migration.oldid1996
dc.contributor.advisorShi, Yan
dc.contributor.authorALGHAMDI, ENAAM
dc.date.accessioned2014-04-11T19:33:22Z
dc.date.available2014-06-16T07:00:29Z
dc.date.issued2014-04-11
dc.date.submitted2014en
dc.description.abstractAntigen recognition requires the communication between T cells and antigen presenting cells (APC) such as dendritic cells (DC). Although there is progress in understanding molecular components at the intercellular contact, the specific role of the physical cross-junctional adhesion between CD4+ T cells and DCs is not fully understood. In this study, we investigate cell-to-cell contact mediated by adhesion molecules between these cells in order to elicit the mechanism involved. Lymphocyte function-associated antigen-1 (LFA-1) adhesion molecule on the surface of T cells interacts with intracellular adhesion molecule-1 (ICAM-1) on the APC. This adhesion pair has been shown to be essential for the contact between the cells. The role of this adhesion pair and its expression and regulation on naïve CD4+ T cells has not been compared to its role on activated T cells. Using atomic force microscopy (AFM) we investigated the increase in interaction forces between naïve CD4+ T cells and DCs. These forces were compared to those involving activated T cells to see the difference between naïve and activated T cells in their adhesion properties with DCs, which involves LFA-1 integrin molecule. Understanding the role of LFA-1 interaction with ICAM-1 provides further insight into therapies using CD4+ T cells.en_US
dc.identifier.citationALGHAMDI, ENAAM. (2014). Force Based Comparison Between Naïve and Activated CD4+ T Cells during Interaction with Dendritic Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26206en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26206
dc.identifier.urihttp://hdl.handle.net/11023/1414
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subjectImmunology
dc.subject.classificationT CELLSen_US
dc.subject.classificationDENDRITIC CELLSen_US
dc.subject.classificationCD4+ T CELLSen_US
dc.subject.classificationinteractionsen_US
dc.subject.classificationAtomic Force Microscopyen_US
dc.subject.classificationAFMen_US
dc.subject.classificationLFA-1en_US
dc.titleForce Based Comparison Between Naïve and Activated CD4+ T Cells during Interaction with Dendritic Cells
dc.typemaster thesis
thesis.degree.disciplineMicrobiology & Infectious Diseases
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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