Characterization of Transforming Acidic Coiled Coil Protein Three, a Protein Phosphatase One Binding Protein

Simple item page
dc.contributor.advisorMoorhead, Greg
dc.contributor.authorRackel, Brooke
dc.contributor.committeememberBonni, Shirin
dc.contributor.committeememberCobb, Jennifer
dc.contributor.committeememberZaremberg, Vanina
dc.date2018-02
dc.date.accessioned2018-01-19T00:45:53Z
dc.date.available2018-01-19T00:45:53Z
dc.date.issued2018-01-08
dc.description.abstractProtein phosphorylation is a key signaling mechanism utilized by cells as a means of regulating various pathways within the cell. Protein phosphatase one (PP1) is a highly conserved enzyme that catalyzes the removal of a phosphate group from serine/threonine residues in eukaryotes. PP1 gains substrate specificity through the binding of regulatory proteins, of which over 200 are currently identified in humans. Most of these subunits dock PP1 via the RVxF binding motif. Transforming acidic coiled coil protein three (TACC3) has been identified as a potential PP1 interactor through quantitative mass spectrometry and also contains an RVxF motif (KVTF). Here, I have validated the TACC3-PP1 interaction, and demonstrated that it occurs via the RVxF motif. TACC3 is a non-motor spindle assembly protein and plays an important role in the proper segregation of chromosomes during mitosis. Due to its key role in maintaining genomic integrity, it has recently been implicated in a number of cancers. Here, I explore the expression of TACC3 mRNA in 20 cancers and the effect of aberrant TACC3 expression on patient survival from clinical data. The work presented here increases our knowledge of PP1 and its interaction with the regulatory subunit TACC3, and also provides insight into the role TACC3 is playing in cancer.en_US
dc.identifier.citationRackel, B.D. (2017). Characterization of Transforming Acidic Coiled Coil Protein Three, a Protein Phosphatase One Binding Protein (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca.en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/5358
dc.identifier.urihttp://hdl.handle.net/1880/106277
dc.language.isoenen_US
dc.publisher.facultyScienceen_US
dc.publisher.institutionUniversity of Calgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.en_US
dc.subject.classificationBiochemistryen_US
dc.titleCharacterization of Transforming Acidic Coiled Coil Protein Three, a Protein Phosphatase One Binding Proteinen_US
dc.typemaster thesisen_US
thesis.degree.disciplineBiological Sciencesen_US
thesis.degree.grantorUniversity of Calgaryen_US
thesis.degree.nameMaster of Science (MSc)en_US
ucalgary.item.requestcopytrue
ucalgary.thesis.checklistI confirm that I have submitted all of the required forms to Faculty of Graduate Studies.en_US
ucalgary.thesis.laclicenseLibraries and Archives Canada THESES NON-EXCLUSIVE LICENSE I hereby grant a non-exclusive license, for the full term of copyright protection, to Library and Archives Canada: to preserve, perform, produce, reproduce, translate theses and dissertations in any format, and to make available in print or online by telecommunication to the public for non-commercial purposes. I undertake to submit my thesis, through my university, to Library and Archives Canada. Any abstract submitted with the thesis will be considered to form part of the thesis. I represent and promise that my thesis is my original work, does not infringe any rights of others, and that I have the right to make the grant conferred by this non-exclusive license. If third party copyrighted material was included in my thesis for which, under the terms of the Copyright Act, written permission from the copyright owners is required I have obtained such permission from the copyright owners to do the acts mentioned in paragraph (a) above for the full term of copyright protection I retain copyright ownership and moral rights in my thesis, and may deal with the copyright in my thesis, in any way consistent with rights granted by me to Library and Archives Canada in this non-exclusive license. I further promise to inform any person to whom I may hereafter assign or license my copyright in my thesis of the rights granted by me to Library and Archives Canada in this non-exclusive license.en_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ucalgary_2018_rackel_brooke.pdf
Size:
7.97 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Open Theses and Dissertations