Investigating the regulatory roles of Platelet-derived Growth Factor in the dermal stem cell niche

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atmire.migration.oldid1301
dc.contributor.advisorBiernaskie, Jeff
dc.contributor.authorMoffatt, Garrett Evan
dc.date.accessioned2013-09-13T20:41:09Z
dc.date.available2015-03-13T07:00:25Z
dc.date.issued2013-09-13
dc.date.submitted2013en
dc.description.abstractSkin-derived Precursors (SKPs) are multipotent, self-renewing adult dermal stem cells able to differentiate into functional neural and mesenchymal progeny. SKPs reside within a specialized niche including both the hair follicle dermal papilla and dermal sheath and function to induce hair follicle morphogenesis and cyclic regeneration. However, the factors regulating their behavior are not understood. My work demonstrates that Platelet-derived Growth Factor (PDGF) is a key regulator of SKP function within their hair follicle niche. Using adult SKPs I show that growth in the presence of PDGF-B promotes increased SKP proliferation and self-renewal in vitro, as well as increased hair follicle formation in an ex vivo hair growth assay. Finally, my work identifies the hair follicle epithelium (outer root sheath) and potentially dermal adipocytes as a source of PDGF in the skin. Understanding this regulation will improve our ability to expand SKP numbers and quality for therapeutic application following skin injury.en_US
dc.description.embargoterms18 monthsen_US
dc.identifier.citationMoffatt, G. E. (2013). Investigating the regulatory roles of Platelet-derived Growth Factor in the dermal stem cell niche (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26646en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26646
dc.identifier.urihttp://hdl.handle.net/11023/962
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectCell
dc.subject.classificationSkinen_US
dc.subject.classificationHairen_US
dc.subject.classificationStem Cellsen_US
dc.titleInvestigating the regulatory roles of Platelet-derived Growth Factor in the dermal stem cell niche
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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