Interaction of Silica Nanoparticles with Primary Alveolar Epithelial Cells

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atmire.migration.oldid3516
dc.contributor.advisorAmrein, Matthias
dc.contributor.authorBogari, Nawaf N.
dc.date.accessioned2015-09-01T16:19:20Z
dc.date.embargolift2017-08-31T16:19:20Z
dc.date.issued2015-09-01
dc.date.submitted2015en
dc.description.abstractThe fate of nanoparticles reaching the alveolar lung is not completely understood. Clearance of these particles has been ascribed to the alveolar macrophages scavenging this region. In contrast, in my thesis, I show in vitro, that particles interact more strongly with the alveolar epithelial cells (AECs) than alveolar macrophages (AMs), are then taken up efficiently and transported across the cells and released on the basolateral side. First, I developed a protocol for obtaining a pure culture of rat AEC. Single cell force spectroscopy showed the AEC to respond strongly and in a clathrin-independent manner to the particles, unlike a host of control cells, including alveolar macrophages. Fluorescence light microscopy and total internal reflection fluorescence light microscopy (TIRF) demonstrated the transport to be novel, actin-dependent and microtubule independent. In summary, my thesis provides evidence of a second clearance mechanism in addition to AMs.en_US
dc.description.embargoterms2 yearsen_US
dc.identifier.citationBogari, N. N. (2015). Interaction of Silica Nanoparticles with Primary Alveolar Epithelial Cells (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26596en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26596
dc.identifier.urihttp://hdl.handle.net/11023/2417
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectEducation--Health
dc.subjectBiology--Cell
dc.subjectPhysiology
dc.subjectHealth Sciences
dc.subjectImmunology
dc.subject.classificationNanoparticlesen_US
dc.subject.classificationAlveolar epithelial cellsen_US
dc.subject.classificationPrimary cell cultureen_US
dc.subject.classificationEndocytosisen_US
dc.titleInteraction of Silica Nanoparticles with Primary Alveolar Epithelial Cells
dc.typemaster thesis
thesis.degree.disciplineCardiovascular & Respiratory Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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