Expansion of Skin-Derived Precursor Cells (SKPs) in Stirred Suspension Bioreactors

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atmire.migration.oldid4353
dc.contributor.advisorKallos, Michael
dc.contributor.authorBoon, Kathryn
dc.contributor.committeememberBiernaskie, Jeffrey
dc.contributor.committeememberUngrin, Mark
dc.contributor.committeememberHart, David
dc.contributor.committeememberDe la Hoz Siegler, Hector
dc.date.accessioned2016-05-02T20:55:53Z
dc.date.available2016-05-02T20:55:53Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractSkin-derived precursor cells (SKPs) have potential therapeutic applications for dermal regeneration in patients who have undergone split-thickness skin graft (STSG) surgeries due to severe burns. While these cells are traditionally grown in vitro in static t-flasks, a more standardized, well-controlled culture environment is desirable so that sufficient numbers of cells can be produced for clinical applications. This study outlines a bioprocess which could be used to expand SKPs, and establishes a baseline of how these cells grow in static and bioreactor conditions. Using a 9% total body surface area burn as the baseline, it was determined that a fold expansion of approximately 45 to 270 would be required for a 10 cm² piece of donor skin, assuming all cells isolated proliferate and are of therapeutic benefit. While this is not feasible based on current expansion achieved in bioreactors, this study provides useful information moving forward towards more advanced bioprocess development.en_US
dc.identifier.citationBoon, K. (2016). Expansion of Skin-Derived Precursor Cells (SKPs) in Stirred Suspension Bioreactors (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26042en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26042
dc.identifier.urihttp://hdl.handle.net/11023/2927
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology--Cell
dc.subjectMedicine and Surgery
dc.subjectEngineering--Biomedical
dc.subjectEngineering--Chemical
dc.subject.classificationskin-derived precursorsen_US
dc.subject.classificationbioreactorsen_US
dc.subject.classificationbioprocessen_US
dc.subject.classificationskin graftsen_US
dc.titleExpansion of Skin-Derived Precursor Cells (SKPs) in Stirred Suspension Bioreactors
dc.typemaster thesis
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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