The use of natural genetic variation in Caenorhabditis elegans to identify novel polymorphisms that can confer benzimidazole resistance in nematodes

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atmire.migration.oldid2234
dc.contributor.advisorGilleard, John
dc.contributor.authorLatheef, Sharmilah Luthfia Jezmine
dc.date.accessioned2014-06-12T14:57:52Z
dc.date.available2014-11-17T08:00:32Z
dc.date.issued2014-06-12
dc.date.submitted2014en
dc.description.abstractBenzimidazoles are important drugs for parasite control. Benzimidazole resistance is widespread in parasites of domestic animals, and an emerging problem in human parasites. Caenorhabditis elegans is a powerful model system to study biology of drug resistance. Using natural genetic variation in wild populations of the non-parasitic nematode C. elegans, three novel amino acid substitutions and a deletion in the β-tubulin drug target have been identified that confer varying levels of benzimidazole resistance. These residues are different to those previously reported in other organisms including nematode parasites or fungi and provide new candidate polymorphisms to be investigated in parasitic nematode species including human parasites where resistance is poorly understood. They may also represent new residues important for drug binding. In addition, presence of these resistance conferring polymorphisms in wild populations of a free-living nematode may indicate benzimidazole drug residues in the environment having a significant impact on natural fauna. Finally, the presence of null ben-1 β-tubulin alleles in wild C. elegans populations indicates the functional redundancy of this gene in nature.en_US
dc.identifier.citationLatheef, S. L. (2014). The use of natural genetic variation in Caenorhabditis elegans to identify novel polymorphisms that can confer benzimidazole resistance in nematodes (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28412en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28412
dc.identifier.urihttp://hdl.handle.net/11023/1575
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.facultyVeterinary Medicine
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectGenetics
dc.subject.classificationBenzimidazole resistanceen_US
dc.subject.classificationTubulinen_US
dc.subject.classificationMutationsen_US
dc.subject.classificationGenetic variationen_US
dc.subject.classificationC.elegansen_US
dc.titleThe use of natural genetic variation in Caenorhabditis elegans to identify novel polymorphisms that can confer benzimidazole resistance in nematodes
dc.typemaster thesis
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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