The Effects of General Anesthetics on Neuron Structure and Function

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atmire.migration.oldid4569
dc.contributor.advisorSyed, Naweed Imam
dc.contributor.authorArmstrong, Ryden J
dc.contributor.committeememberXu, Fenglian
dc.contributor.committeememberShutt, Timothy
dc.contributor.committeememberDobson, Gary
dc.contributor.committeememberShearer, Jane
dc.date.accessioned2016-07-05T17:12:34Z
dc.date.available2016-07-05T17:12:34Z
dc.date.issued2016
dc.date.submitted2016en
dc.description.abstractRecent studies have implicated general anesthetic exposure to long-lasting cognitive impairments in children. However, the mechanisms that underlie anesthetic toxicity remain controversial. Here, I used an in vitro primary neuron culture to determine the effects of two volatile general anesthetics – sevoflurane and desflurane – on neuron development. To this end, I first quantified how general anesthetics affect neuron structural development. I show that sevoflurane and desflurane impair neurite growth, neuron polarization, and mitochondrial morphology. Next, I determined the functional impact of general anesthetics on neuronal synaptogenesis and spontaneous synaptic activity. I demonstrate that both sevoflurane and desflurane impair synaptogenesis and spontaneous synaptic current frequency. Finally, I examined a promising mitochondrial protective compound – Mdivi-1 – and found it to reverse many of the noxious effects of sevoflurane and desflurane. These results show, for the first time, several subcellular impacts of sevoflurane and desflurane, which may underlie the learning and memory deficits seen clinically.en_US
dc.identifier.citationArmstrong, R. J. (2016). The Effects of General Anesthetics on Neuron Structure and Function (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/28104en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/28104
dc.identifier.urihttp://hdl.handle.net/11023/3100
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectNeuroscience
dc.subjectMedicine and Surgery
dc.subject.classificationGeneral Anestheticsen_US
dc.subject.classificationAnesthetic Neurotoxicityen_US
dc.titleThe Effects of General Anesthetics on Neuron Structure and Function
dc.typemaster thesis
thesis.degree.disciplineNeuroscience
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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