Proteomic analysis of yeast membranes enriched in acyltransferases Gpt2p and Sct1p provides insight into their roles and regulation

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atmire.migration.oldid6018
dc.contributor.advisorZaremberg, Vanina
dc.contributor.authorShabits, Brittney
dc.contributor.committeememberMoorhead, Gregory
dc.contributor.committeememberHansen, David
dc.contributor.committeememberGrewal, Savraj
dc.date.accessioned2017-09-21T22:11:24Z
dc.date.available2017-09-21T22:11:24Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractGpt2p and Sct1p are yeast glycerol-3-phosphate acyltransferases (GPATs) that catalyze the first committed and rate-limiting step in the synthesis of glycerolipids. Although both enzymes catalyze the same reaction, they contribute differentially to downstream steps in the de novo biosynthesis of glycerolipids. Membranes enriched in either Gpt2p or Sct1p were affinity isolated and associated proteins were identified by mass spectrometry. Unique protein profiles were identified for both GPATs, with significant enrichments in proteins related to glycerophospholipid, sphingolipid, and fatty acid metabolism. Identified proteins revealed a hub around palmitoyl-CoA, implying that there is competition between these pathways for the channelling of this fatty acid into specific metabolic branches. Interestingly, a Gpt2p mutant missing its serine-rich C-terminus did not associate with selected proteins found in the wildtype Gpt2p-enriched membranes. This suggests that this protein region of high phosphorylation in Gpt2p may be important in facilitating some protein-protein associations detected in this study.en_US
dc.identifier.citationShabits, B. (2017). Proteomic analysis of yeast membranes enriched in acyltransferases Gpt2p and Sct1p provides insight into their roles and regulation (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/27471en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/27471
dc.identifier.urihttp://hdl.handle.net/11023/4122
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectBiology
dc.subjectBiochemistry
dc.subject.otherProteomics
dc.subject.otherGlycerol-3-phosphate acyltransferase
dc.subject.otherAffinity purification
dc.subject.otherMass spectrometry
dc.titleProteomic analysis of yeast membranes enriched in acyltransferases Gpt2p and Sct1p provides insight into their roles and regulation
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
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