Methanogenic and Aerobic Biodegradation of Model Polycyclic Aromatic Compounds Associated with Canadian Oil Sands

Simple item page
atmire.migration.oldid6183
dc.contributor.advisorGieg, Lisa
dc.contributor.advisorHubert, Casey
dc.contributor.authorMontoya, Oscar
dc.contributor.committeememberVoordouw, Gerrit
dc.contributor.committeememberLarter, Stephen
dc.contributor.committeememberStrous, Marc
dc.date.accessioned2017-11-22T16:38:39Z
dc.date.available2017-11-22T16:38:39Z
dc.date.issued2017
dc.date.submitted2017en
dc.description.abstractPolycyclic aromatic compounds (PACs) are ubiquitous molecules that can be of high importance to remediate due to their potential negative health and environmental effects. The present study used Canadian Oil Sands-derived microbial consortia established methanogenically or aerobically and amended with phenanthrene, dibenzothiophene (DBT), or 2,6-dimethylnaphthalene (2,6-diMN) as sole carbon and energy sources under three salinities. Methane formation was statistically higher in PAC-amended treatments relative to unamended controls under brackish conditions. A fumarate addition metabolite was tentatively detected in incubations amended with 2,6-diMN. DBT was degraded with concomitant methane formation, the first report of this metabolism. 16S rRNA gene sequencing revealed the dominance of methanogens and known PACs degraders. Genus Smithella was only detected in DBT-amended incubations in relatively high abundances suggesting its role in DBT degradation anaerobically. Aerobic degradation of PHEN and 2,6-diMN was also observed. Time-course experiments showed faster PHEN degradation under saline conditions and that the microbial communities were dominated by Janibacter sp, which was also isolated. This research shows that microbial communities from bitumen-impacted environments have the natural ability to degrade PACs, with potential applications in bioremediation.en_US
dc.identifier.citationMontoya, O. (2017). Methanogenic and Aerobic Biodegradation of Model Polycyclic Aromatic Compounds Associated with Canadian Oil Sands (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/26890en_US
dc.identifier.doihttp://dx.doi.org/10.11575/PRISM/26890
dc.identifier.urihttp://hdl.handle.net/11023/4246
dc.language.isoeng
dc.publisher.facultyGraduate Studies
dc.publisher.institutionUniversity of Calgaryen
dc.publisher.placeCalgaryen
dc.rightsUniversity of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.
dc.subjectMicrobiology
dc.subject.otherMethanogeniesis
dc.subject.otherPolycyclic aromatic compounds
dc.subject.otherDibenzothiophene
dc.subject.otherPhenanthrene
dc.subject.other2,6-Dimethylnaphthalene
dc.subject.otherBiodegradation
dc.subject.otherBioremediation
dc.subject.otherAerobic
dc.subject.otherBitumen
dc.subject.otherOil sands
dc.subject.otherSalinities
dc.titleMethanogenic and Aerobic Biodegradation of Model Polycyclic Aromatic Compounds Associated with Canadian Oil Sands
dc.typemaster thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorUniversity of Calgary
thesis.degree.nameMaster of Science (MSc)
ucalgary.item.requestcopytrue
Files
Collections
Open Theses and Dissertations