Effect of the human serum albumin concentration on the metabolism of cisplatin in vitro
| atmire.migration.oldid | 2121 | |
| dc.contributor.advisor | Gailer, Jürgen | |
| dc.contributor.author | Morris, Thomas | |
| dc.date.accessioned | 2014-05-14T22:24:39Z | |
| dc.date.available | 2014-06-16T07:00:43Z | |
| dc.date.issued | 2014-05-14 | |
| dc.date.submitted | 2014 | en |
| dc.description.abstract | Size-exclusion chromatography (SEC) coupled to an inductively coupled plasma atomic emission spectrometer (ICP-AES) was employed to study the effect of the human serum albumin (HSA) concentration on the metabolism of cisplatin (CP) in human blood plasma in vitro. Plasma from 14 healthy adults and 11 pediatric cancer patients was spiked with CP and the Pt distribution was determined at the 5 min and 2 hr time point. The results revealed that as the HSA concentration decreased, the percent of Pt-bound to HSA decreased; whereas the percent of Pt-hydrolysis products increased, while other Pt-species were largely unaffected. These changes were corroborated by fortifying the cancer patient plasma with HSA to healthy concentrations followed by the addition of CP. In summary, these findings imply that the infusion of cancer patients with HSA to healthy plasma levels may decrease the fraction of free hydrolysis products, which may alleviate the severe toxic side effects of CP. | en_US |
| dc.identifier.citation | Morris, T. (2014). Effect of the human serum albumin concentration on the metabolism of cisplatin in vitro (Master's thesis, University of Calgary, Calgary, Canada). Retrieved from https://prism.ucalgary.ca. doi:10.11575/PRISM/25813 | en_US |
| dc.identifier.doi | http://dx.doi.org/10.11575/PRISM/25813 | |
| dc.identifier.uri | http://hdl.handle.net/11023/1523 | |
| dc.language.iso | eng | |
| dc.publisher.faculty | Graduate Studies | |
| dc.publisher.institution | University of Calgary | en |
| dc.publisher.place | Calgary | en |
| dc.rights | University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. | |
| dc.subject | Oncology | |
| dc.subject | Chemistry--Analytical | |
| dc.subject.classification | Oncology | en_US |
| dc.subject.classification | Albumin | en_US |
| dc.subject.classification | cisplatin | en_US |
| dc.subject.classification | Chemotherapy | en_US |
| dc.subject.classification | Metabolism | en_US |
| dc.title | Effect of the human serum albumin concentration on the metabolism of cisplatin in vitro | |
| dc.type | master thesis | |
| thesis.degree.discipline | Chemistry | |
| thesis.degree.grantor | University of Calgary | |
| thesis.degree.name | Master of Science (MSc) | |
| ucalgary.item.requestcopy | true |